LOCUS       BBa_J06101               884 bp    DNA     linear       26-Dec-2024
DEFINITION  Tet repressible lasI generator ( LVA+ )
ACCESSION   BBa_J06101
VERSION     BBa_J06101.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..683
                     /label=LasI
     misc_feature    684..716
                     /label=LVA
     misc_feature    723..747
                     /label=Help:Barcodes
     CDS             81..722
                     /label=BBa_C0078
     stem_loop       767..810
                     /label=stem_loop
     misc_feature    756..835
                     /label=BBa_B0010
     terminator      756..835
                     /label=BBa_B0010
     stem_loop       851..870
                     /label=T7 TE
     misc_feature    877..877
                     /label=stop
     polyA_site      871..884
                     /label=polya
     misc_feature    844..884
                     /label=BBa_B0012
     terminator      844..884
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcgttc agatcggtcg tcgtgaagag ttcgacaaaa
      121 aactgctggg tgaaatgcac aaactgcgtg ctcaggtttt caaagaacgt aaaggttggg
      181 acgtttccgt tatcgacgaa atggaaatcg acggttacga cgctctgtcc ccgtactaca
      241 tgctgatcca ggaagacacc ccggaagctc aggttttcgg ttgctggcgt atcttcgaca
      301 ccaccggtcc gtacatgctg aaaaacacct tcccggaact gctgcacggt aaagaagctc
      361 cgtgctcccc gcacatctgg gaactgtccc gtttcgctat caactccggt cagaaaggtt
      421 ccctgggttt ctccgactgc accctggaag ctatgcgtgc tctggctcgt tactccttgc
      481 agaacgacat ccagaccctg gttaccgtta ccaccgttgg tgttgaaaaa atgatgatcc
      541 gtgctggtct ggacgtttcc cgtttcggtc cgcacctgaa aatcggtatc gaacgtgctg
      601 ttgctctgcg tatcgaactg aacgctaaaa cccagatcgc tctgtacggt ggtgttctgg
      661 ttgaacagcg tctggctgtt tccgctgcta acgacgaaaa ctacgctctg gttgcttaat
      721 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc
      781 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      841 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//