LOCUS       BBa_J06102               826 bp    DNA     linear       22-Nov-2024
DEFINITION  Tet repressible lasI generator ( LVA- )
ACCESSION   BBa_J06102
VERSION     BBa_J06102.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..689
                     /label=lasI
     CDS             81..689
                     /label=BBa_C0178
     stem_loop       709..752
                     /label=stem_loop
     misc_feature    698..777
                     /label=BBa_B0010
     terminator      698..777
                     /label=BBa_B0010
     stem_loop       793..812
                     /label=T7 TE
     misc_feature    819..819
                     /label=stop
     polyA_site      813..826
                     /label=polya
     misc_feature    786..826
                     /label=BBa_B0012
     terminator      786..826
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcgttc agatcggtcg tcgtgaagag ttcgacaaaa
      121 aactgctggg tgaaatgcac aaactgcgtg ctcaggtttt caaagaacgt aaaggttggg
      181 acgtttccgt tatcgacgaa atggaaatcg acggttacga cgctctgtcc ccgtactaca
      241 tgctgatcca ggaagacacc ccggaagctc aggttttcgg ttgctggcgt atcttcgaca
      301 ccaccggtcc gtacatgctg aaaaacacct tcccggaact gctgcacggt aaagaagctc
      361 cgtgctcccc gcacatctgg gaactgtccc gtttcgctat caactccggt cagaaaggtt
      421 ccctgggttt ctccgactgc accctggaag ctatgcgtgc tctggctcgt tactccttgc
      481 agaacgacat ccagaccctg gttaccgtta ccaccgttgg tgttgaaaaa atgatgatcc
      541 gtgctggtct ggacgtttcc cgtttcggtc cgcacctgaa aatcggtatc gaacgtgctg
      601 ttgctctgcg tatcgaactg aacgctaaaa cccagatcgc tctgtacggt ggtgttctgg
      661 ttgaacagcg tctggctgtt tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//