LOCUS BBa_J06104 2088 bp DNA linear 22-Nov-2024 DEFINITION 3OC12HSL receiver, CI repressor hybrid ACCESSION BBa_J06104 VERSION BBa_J06104.1 FEATURES Location/Qualifiers promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=P(Cat) promoter 1..38 /label=BBa_I14033 misc_feature 51..54 /label=conserved RBS 47..58 /label=BBa_B0034 CDS 65..781 /label=lasR misc_feature 782..814 /label=LVA misc_feature 544..544 /label=G misc_feature 821..845 /label=Help:Barcodes CDS 65..820 /label=BBa_C0079 stem_loop 865..908 /label=stem_loop misc_feature 854..933 /label=BBa_B0010 terminator 854..933 /label=BBa_B0010 stem_loop 949..968 /label=T7 TE misc_feature 975..975 /label=stop polyA_site 969..982 /label=polya misc_feature 942..982 /label=BBa_B0012 terminator 942..982 /label=BBa_B0012 misc_feature 1036..1053 /label=OP2 misc_feature 1096..1115 /label=OP1 promoter 1107..1112 /label=-35 promoter 1130..1135 /label=-10 promoter 991..1147 /label=BBa_R0079 misc_feature 1..1147 /label=BBa_J06400 RBS 1156..1170 /label=RBS-1\Strong RBS 1163..1167 /label=RBS misc_feature 1156..1170 /label=BBa_B0030 RBS 1156..1170 /label=BBa_B0030 CDS 1180..1887 /label=cI lambda misc_feature 1888..1920 /label=LVA misc_feature 1927..1951 /label=Help:Barcodes CDS 1177..1951 /label=BBa_C0051 stem_loop 1971..2014 /label=stem_loop misc_feature 1960..2039 /label=BBa_B0010 terminator 1960..2039 /label=BBa_B0010 stem_loop 2055..2074 /label=T7 TE misc_feature 2081..2081 /label=stop polyA_site 2075..2088 /label=polya misc_feature 2048..2088 /label=BBa_B0012 terminator 2048..2088 /label=BBa_B0012 terminator 1960..2088 /label=BBa_B0015 ORIGIN 1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata 61 ctagatggcc ttggttgacg gttttcttga gctggaacgc tcaagtggaa aattggagtg 121 gagcgccatc ctccagaaga tggcgagcga ccttggattc tcgaagatcc tgttcggcct 181 gttgcctaag gacagccagg actacgagaa cgccttcatc gtcggcaact acccggccgc 241 ctggcgcgag cattacgacc gggctggcta cgcgcgggtc gacccgacgg tcagtcactg 301 tacccagagc gtactgccga ttttctggga accgtccatc taccagacgc gaaagcagca 361 cgagttcttc gaggaagcct cggccgccgg cctggtgtat gggctgacca tgccgctgca 421 tggtgctcgc ggcgaactcg gcgcgctgag cctcagcgtg gaagcggaaa accgggccga 481 ggccaaccgt ttcatagagt cggtcctgcc gaccctgtgg atgctcaagg actacgcact 541 gcaaagcggt gccggactgg ccttcgaaca tccggtcagc aaaccggtgg ttctgaccag 601 ccgggagaag gaagtgttgc agtggtgcgc catcggcaag accagttggg agatatcggt 661 tatctgcaac tgctcggaag ccaatgtgaa cttccatatg ggaaatattc ggcggaagtt 721 cggtgtgacc tcccgccgcg tagcggccat tatggccgtt aatttgggtc ttattactct 781 cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag 841 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 901 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 961 ggtgggcctt tctgcgttta tatactagag gcccctcgct gagcgcgtcc cggagctggg 1021 ggcaacctag ctgccacctg cttttctgct agctattcca gcgaaaacat acagatttcc 1081 ggcgaaatca aggctacctg ccagttctgg caggtttggc cgcgggttct ttttggtaca 1141 cgaaagctac tagagattaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta 1201 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 1261 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 1321 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa 1381 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 1441 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 1501 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag 1561 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 1621 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 1681 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 1741 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 1801 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 1861 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct 1921 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa 1981 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2041 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //