LOCUS       BBa_J06117              1791 bp    DNA     linear       22-Dec-2024
ACCESSION   BBa_J06117
VERSION     BBa_J06117.1  
FEATURES             Location/Qualifiers
     misc_feature    1..151
                     /label=R0079
     misc_feature    152..168
                     /label=CI lambda O1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..168
                     /label=BBa_I14016
     RBS             177..191
                     /label=RBS-1\Strong
     RBS             184..188
                     /label=RBS
     misc_feature    177..191
                     /label=BBa_B0030
     RBS             177..191
                     /label=BBa_B0030
     CDS             198..959
                     /label=YFP (LVA)
     misc_feature    954..959
                     /label=2
     misc_feature    915..953
                     /label=SsrA
     misc_feature    439..439
                     /label=A (Q->L)
     misc_feature    198..959
                     /label=BBa_E0032
     misc_feature    198..959
                     /label=BBa_E0032
     misc_feature    974..977
                     /label=conserved
     RBS             968..981
                     /label=BBa_B0031
     CDS             988..1590
                     /label=LasI
     misc_feature    1591..1623
                     /label=LVA
     misc_feature    1630..1654
                     /label=Help:Barcodes
     CDS             988..1629
                     /label=BBa_C0078
     stem_loop       1674..1717
                     /label=stem_loop
     misc_feature    1663..1742
                     /label=BBa_B0010
     terminator      1663..1742
                     /label=BBa_B0010
     stem_loop       1758..1777
                     /label=T7 TE
     misc_feature    1784..1784
                     /label=stop
     polyA_site      1778..1791
                     /label=polya
     misc_feature    1751..1791
                     /label=BBa_B0012
     terminator      1751..1791
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca ctacctctgg cggtgatata ctagagatta
      181 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      241 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      301 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      361 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct
      421 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      481 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      541 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      601 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      661 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      721 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      781 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
      841 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      901 acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat
      961 actagagtca cacaggaaac ctactagatg atcgttcaga tcggtcgtcg tgaagagttc
     1021 gacaaaaaac tgctgggtga aatgcacaaa ctgcgtgctc aggttttcaa agaacgtaaa
     1081 ggttgggacg tttccgttat cgacgaaatg gaaatcgacg gttacgacgc tctgtccccg
     1141 tactacatgc tgatccagga agacaccccg gaagctcagg ttttcggttg ctggcgtatc
     1201 ttcgacacca ccggtccgta catgctgaaa aacaccttcc cggaactgct gcacggtaaa
     1261 gaagctccgt gctccccgca catctgggaa ctgtcccgtt tcgctatcaa ctccggtcag
     1321 aaaggttccc tgggtttctc cgactgcacc ctggaagcta tgcgtgctct ggctcgttac
     1381 tccttgcaga acgacatcca gaccctggtt accgttacca ccgttggtgt tgaaaaaatg
     1441 atgatccgtg ctggtctgga cgtttcccgt ttcggtccgc acctgaaaat cggtatcgaa
     1501 cgtgctgttg ctctgcgtat cgaactgaac gctaaaaccc agatcgctct gtacggtggt
     1561 gttctggttg aacagcgtct ggctgtttcc gctgctaacg acgaaaacta cgctctggtt
     1621 gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat caaataaaac
     1681 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1741 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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