##gff-version 3
##sequence-region BBa_J06127 1 1790
BBa_J06127	.	terminator	1750	1790	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06127	.	stem_loop	1757	1776	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06127	.	engineered_region	1750	1790	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06127	.	stop_codon	1783	1783	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06127	.	polyA_site	1777	1790	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06127	.	terminator	1662	1741	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06127	.	engineered_region	1662	1741	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06127	.	stem_loop	1673	1716	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06127	.	engineered_region	198	959	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J06127	.	engineered_region	198	959	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J06127	.	sequence_alteration	439	439	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J06127	.	CDS	198	959	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J06127	.	stop_codon	954	959	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J06127	.	tag	915	953	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J06127	.	ribosome_entry_site	968	980	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J06127	.	engineered_region	968	980	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J06127	.	ribosome_entry_site	974	977	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J06127	.	ribosome_entry_site	968	980	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J06127	.	ribosome_entry_site	177	191	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J06127	.	engineered_region	177	191	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J06127	.	ribosome_entry_site	184	188	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J06127	.	ribosome_entry_site	177	191	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J06127	.	CDS	987	1628	.	+	0	ID=BBa_C0078;Name=BBa_C0078
BBa_J06127	.	tag	1590	1622	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_J06127	.	CDS	987	1589	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_J06127	.	engineered_tag	1629	1653	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_J06127	.	promoter	1	168	.	+	0	ID=BBa_I14016;Name=BBa_I14016
BBa_J06127	.	engineered_region	1	151	.	+	0	ID=annotation1017859;Name=R0079;Parent=BBa_I14016
BBa_J06127	.	promoter	140	145	.	+	0	ID=annotation1026125;Name=-10;Parent=BBa_I14016
BBa_J06127	.	non_covalent_binding_site	152	168	.	+	0	ID=annotation1017878;Name=CI lambda O1;Parent=BBa_I14016
BBa_J06127	.	promoter	117	122	.	+	0	ID=annotation1026124;Name=-35;Parent=BBa_I14016
>BBa_J06127
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacactacctct
ggcggtgatatactagagattaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgc
ccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccaccta
cggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggc
tacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggct
acgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacac
cctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaac
tacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaaca
tcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccga
caaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttc
gtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttag
tagcttaataatactagagtcacacaggaaagtactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaa
actgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaa
atggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcg
gttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagc
tccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgac
tgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccg
ttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacg
tgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctg
gctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata