LOCUS BBa_J06128 1729 bp DNA linear 25-Nov-2024 ACCESSION BBa_J06128 VERSION BBa_J06128.1 FEATURES Location/Qualifiers misc_feature 1..151 /label=R0079 misc_feature 152..168 /label=CI lambda O1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..168 /label=BBa_I14016 misc_feature 181..184 /label=conserved RBS 177..188 /label=BBa_B0034 CDS 195..956 /label=YFP (LVA) misc_feature 951..956 /label=2 misc_feature 912..950 /label=SsrA misc_feature 436..436 /label=A (Q->L) misc_feature 195..956 /label=BBa_E0032 misc_feature 195..956 /label=BBa_E0032 RBS 965..977 /label=RBS-3\Weak RBS 971..974 /label=RBS misc_feature 965..977 /label=BBa_B0032 RBS 965..977 /label=BBa_B0032 CDS 984..1592 /label=lasI CDS 984..1592 /label=BBa_C0178 stem_loop 1612..1655 /label=stem_loop misc_feature 1601..1680 /label=BBa_B0010 terminator 1601..1680 /label=BBa_B0010 stem_loop 1696..1715 /label=T7 TE misc_feature 1722..1722 /label=stop polyA_site 1716..1729 /label=polya misc_feature 1689..1729 /label=BBa_B0012 terminator 1689..1729 /label=BBa_B0012 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca ctacctctgg cggtgatata ctagagaaag 181 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 241 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 301 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 361 tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc 421 ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 481 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 541 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 601 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 661 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 721 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 781 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc 841 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 901 agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact 961 agagtcacac aggaaagtac tagatgatcg ttcagatcgg tcgtcgtgaa gagttcgaca 1021 aaaaactgct gggtgaaatg cacaaactgc gtgctcaggt tttcaaagaa cgtaaaggtt 1081 gggacgtttc cgttatcgac gaaatggaaa tcgacggtta cgacgctctg tccccgtact 1141 acatgctgat ccaggaagac accccggaag ctcaggtttt cggttgctgg cgtatcttcg 1201 acaccaccgg tccgtacatg ctgaaaaaca ccttcccgga actgctgcac ggtaaagaag 1261 ctccgtgctc cccgcacatc tgggaactgt cccgtttcgc tatcaactcc ggtcagaaag 1321 gttccctggg tttctccgac tgcaccctgg aagctatgcg tgctctggct cgttactcct 1381 tgcagaacga catccagacc ctggttaccg ttaccaccgt tggtgttgaa aaaatgatga 1441 tccgtgctgg tctggacgtt tcccgtttcg gtccgcacct gaaaatcggt atcgaacgtg 1501 ctgttgctct gcgtatcgaa ctgaacgcta aaacccagat cgctctgtac ggtggtgttc 1561 tggttgaaca gcgtctggct gtttcctaat aatactagag ccaggcatca aataaaacga 1621 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1681 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //