LOCUS       BBa_J06132               825 bp    DNA     linear       25-Nov-2024
ACCESSION   BBa_J06132
VERSION     BBa_J06132.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..73
                     /label=RBS-4\Weaker
     RBS             69..72
                     /label=RBS
     misc_feature    63..73
                     /label=BBa_B0033
     RBS             63..73
                     /label=BBa_B0033
     CDS             80..688
                     /label=lasI
     CDS             80..688
                     /label=BBa_C0178
     stem_loop       708..751
                     /label=stem_loop
     misc_feature    697..776
                     /label=BBa_B0010
     terminator      697..776
                     /label=BBa_B0010
     stem_loop       792..811
                     /label=T7 TE
     misc_feature    818..818
                     /label=stop
     polyA_site      812..825
                     /label=polya
     misc_feature    785..825
                     /label=BBa_B0012
     terminator      785..825
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gactactaga tgatcgttca gatcggtcgt cgtgaagagt tcgacaaaaa
      121 actgctgggt gaaatgcaca aactgcgtgc tcaggttttc aaagaacgta aaggttggga
      181 cgtttccgtt atcgacgaaa tggaaatcga cggttacgac gctctgtccc cgtactacat
      241 gctgatccag gaagacaccc cggaagctca ggttttcggt tgctggcgta tcttcgacac
      301 caccggtccg tacatgctga aaaacacctt cccggaactg ctgcacggta aagaagctcc
      361 gtgctccccg cacatctggg aactgtcccg tttcgctatc aactccggtc agaaaggttc
      421 cctgggtttc tccgactgca ccctggaagc tatgcgtgct ctggctcgtt actccttgca
      481 gaacgacatc cagaccctgg ttaccgttac caccgttggt gttgaaaaaa tgatgatccg
      541 tgctggtctg gacgtttccc gtttcggtcc gcacctgaaa atcggtatcg aacgtgctgt
      601 tgctctgcgt atcgaactga acgctaaaac ccagatcgct ctgtacggtg gtgttctggt
      661 tgaacagcgt ctggctgttt cctaataata ctagagccag gcatcaaata aaacgaaagg
      721 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      781 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//