##gff-version 3
##sequence-region BBa_J06135 1 1092
BBa_J06135	.	engineered_region	194	955	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J06135	.	tag	911	949	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J06135	.	stop_codon	950	955	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J06135	.	sequence_alteration	435	435	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J06135	.	engineered_region	194	955	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J06135	.	CDS	194	955	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J06135	.	terminator	964	1043	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06135	.	stem_loop	975	1018	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06135	.	engineered_region	964	1043	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06135	.	ribosome_entry_site	177	187	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_J06135	.	engineered_region	177	187	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_J06135	.	ribosome_entry_site	183	186	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_J06135	.	ribosome_entry_site	177	187	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_J06135	.	promoter	1	168	.	+	0	ID=BBa_I14016;Name=BBa_I14016
BBa_J06135	.	promoter	117	122	.	+	0	ID=annotation1026124;Name=-35;Parent=BBa_I14016
BBa_J06135	.	promoter	140	145	.	+	0	ID=annotation1026125;Name=-10;Parent=BBa_I14016
BBa_J06135	.	engineered_region	1	151	.	+	0	ID=annotation1017859;Name=R0079;Parent=BBa_I14016
BBa_J06135	.	non_covalent_binding_site	152	168	.	+	0	ID=annotation1017878;Name=CI lambda O1;Parent=BBa_I14016
BBa_J06135	.	terminator	1052	1092	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06135	.	polyA_site	1079	1092	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06135	.	stem_loop	1059	1078	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06135	.	engineered_region	1052	1092	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06135	.	stop_codon	1085	1085	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_J06135
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacactacctct
ggcggtgatatactagagtcacacaggactactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccat
cctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggc
aagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacg
gcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgt
ccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctg
gtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactaca
acagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcga
ggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaac
cactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtga
ccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagc
ttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata