##gff-version 3
##sequence-region BBa_J06137 1 1785
BBa_J06137	.	terminator	1657	1736	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06137	.	engineered_region	1657	1736	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06137	.	stem_loop	1668	1711	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06137	.	promoter	1	168	.	+	0	ID=BBa_I14016;Name=BBa_I14016
BBa_J06137	.	engineered_region	1	151	.	+	0	ID=annotation1017859;Name=R0079;Parent=BBa_I14016
BBa_J06137	.	promoter	117	122	.	+	0	ID=annotation1026124;Name=-35;Parent=BBa_I14016
BBa_J06137	.	promoter	140	145	.	+	0	ID=annotation1026125;Name=-10;Parent=BBa_I14016
BBa_J06137	.	non_covalent_binding_site	152	168	.	+	0	ID=annotation1017878;Name=CI lambda O1;Parent=BBa_I14016
BBa_J06137	.	terminator	1745	1785	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06137	.	engineered_region	1745	1785	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06137	.	stop_codon	1778	1778	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06137	.	stem_loop	1752	1771	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06137	.	polyA_site	1772	1785	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06137	.	ribosome_entry_site	177	188	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06137	.	conserved_region	181	184	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J06137	.	engineered_region	195	956	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J06137	.	stop_codon	951	956	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J06137	.	CDS	195	956	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J06137	.	engineered_region	195	956	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J06137	.	tag	912	950	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J06137	.	sequence_alteration	436	436	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J06137	.	ribosome_entry_site	965	975	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_J06137	.	ribosome_entry_site	971	974	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_J06137	.	ribosome_entry_site	965	975	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_J06137	.	engineered_region	965	975	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_J06137	.	CDS	982	1623	.	+	0	ID=BBa_C0078;Name=BBa_C0078
BBa_J06137	.	CDS	982	1584	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_J06137	.	engineered_tag	1624	1648	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_J06137	.	tag	1585	1617	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
>BBa_J06137
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacactacctct
ggcggtgatatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgccca
tcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacgg
caagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctac
ggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacg
tccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccct
ggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactac
aacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcg
aggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaa
ccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtg
accgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtag
cttaataatactagagtcacacaggactactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgc
tgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatgga
aatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgc
tggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgt
gctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcac
cctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggt
gttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctg
ttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgt
ttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata