##gff-version 3
##sequence-region BBa_J06146 1 959
BBa_J06146	.	engineered_region	198	959	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J06146	.	CDS	198	959	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J06146	.	stop_codon	954	959	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J06146	.	sequence_alteration	439	439	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J06146	.	engineered_region	198	959	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J06146	.	tag	915	953	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J06146	.	ribosome_entry_site	177	191	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J06146	.	ribosome_entry_site	177	191	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J06146	.	ribosome_entry_site	184	188	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J06146	.	engineered_region	177	191	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J06146	.	promoter	1	168	.	+	0	ID=BBa_I14016;Name=BBa_I14016
BBa_J06146	.	promoter	117	122	.	+	0	ID=annotation1026124;Name=-35;Parent=BBa_I14016
BBa_J06146	.	non_covalent_binding_site	152	168	.	+	0	ID=annotation1017878;Name=CI lambda O1;Parent=BBa_I14016
BBa_J06146	.	engineered_region	1	151	.	+	0	ID=annotation1017859;Name=R0079;Parent=BBa_I14016
BBa_J06146	.	promoter	140	145	.	+	0	ID=annotation1026125;Name=-10;Parent=BBa_I14016
>BBa_J06146
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacactacctct
ggcggtgatatactagagattaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgc
ccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccaccta
cggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggc
tacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggct
acgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacac
cctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaac
tacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaaca
tcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccga
caaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttc
gtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttag
tagcttaataa