LOCUS       BBa_J06148              1731 bp    DNA     linear       22-Dec-2024
ACCESSION   BBa_J06148
VERSION     BBa_J06148.1  
FEATURES             Location/Qualifiers
     misc_feature    1..151
                     /label=R0079
     misc_feature    152..168
                     /label=CI lambda O1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..168
                     /label=BBa_I14016
     misc_feature    181..184
                     /label=conserved
     RBS             177..188
                     /label=BBa_B0034
     CDS             195..956
                     /label=YFP (LVA)
     misc_feature    951..956
                     /label=2
     misc_feature    912..950
                     /label=SsrA
     misc_feature    436..436
                     /label=A (Q->L)
     misc_feature    195..956
                     /label=BBa_E0032
     misc_feature    195..956
                     /label=BBa_E0032
     RBS             965..979
                     /label=RBS-1\Strong
     RBS             972..976
                     /label=RBS
     misc_feature    965..979
                     /label=BBa_B0030
     RBS             965..979
                     /label=BBa_B0030
     CDS             986..1594
                     /label=lasI
     CDS             986..1594
                     /label=BBa_C0178
     stem_loop       1614..1657
                     /label=stem_loop
     misc_feature    1603..1682
                     /label=BBa_B0010
     terminator      1603..1682
                     /label=BBa_B0010
     stem_loop       1698..1717
                     /label=T7 TE
     misc_feature    1724..1724
                     /label=stop
     polyA_site      1718..1731
                     /label=polya
     misc_feature    1691..1731
                     /label=BBa_B0012
     terminator      1691..1731
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca ctacctctgg cggtgatata ctagagaaag
      181 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      241 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      301 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      361 tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc
      421 ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      481 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      541 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      601 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      661 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      721 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      781 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
      841 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      901 agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact
      961 agagattaaa gaggagaaat actagatgat cgttcagatc ggtcgtcgtg aagagttcga
     1021 caaaaaactg ctgggtgaaa tgcacaaact gcgtgctcag gttttcaaag aacgtaaagg
     1081 ttgggacgtt tccgttatcg acgaaatgga aatcgacggt tacgacgctc tgtccccgta
     1141 ctacatgctg atccaggaag acaccccgga agctcaggtt ttcggttgct ggcgtatctt
     1201 cgacaccacc ggtccgtaca tgctgaaaaa caccttcccg gaactgctgc acggtaaaga
     1261 agctccgtgc tccccgcaca tctgggaact gtcccgtttc gctatcaact ccggtcagaa
     1321 aggttccctg ggtttctccg actgcaccct ggaagctatg cgtgctctgg ctcgttactc
     1381 cttgcagaac gacatccaga ccctggttac cgttaccacc gttggtgttg aaaaaatgat
     1441 gatccgtgct ggtctggacg tttcccgttt cggtccgcac ctgaaaatcg gtatcgaacg
     1501 tgctgttgct ctgcgtatcg aactgaacgc taaaacccag atcgctctgt acggtggtgt
     1561 tctggttgaa cagcgtctgg ctgtttccta ataatactag agccaggcat caaataaaac
     1621 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1681 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//