##gff-version 3
##sequence-region BBa_J06148 1 1731
BBa_J06148	.	engineered_region	195	956	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J06148	.	CDS	195	956	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J06148	.	tag	912	950	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J06148	.	engineered_region	195	956	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J06148	.	stop_codon	951	956	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J06148	.	sequence_alteration	436	436	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J06148	.	CDS	986	1594	.	+	0	ID=BBa_C0178;Name=BBa_C0178
BBa_J06148	.	CDS	986	1594	.	+	0	ID=annotation1937124;Name=lasI;Parent=BBa_C0178
BBa_J06148	.	ribosome_entry_site	177	188	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06148	.	conserved_region	181	184	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J06148	.	terminator	1603	1682	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06148	.	stem_loop	1614	1657	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06148	.	engineered_region	1603	1682	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06148	.	promoter	1	168	.	+	0	ID=BBa_I14016;Name=BBa_I14016
BBa_J06148	.	promoter	117	122	.	+	0	ID=annotation1026124;Name=-35;Parent=BBa_I14016
BBa_J06148	.	non_covalent_binding_site	152	168	.	+	0	ID=annotation1017878;Name=CI lambda O1;Parent=BBa_I14016
BBa_J06148	.	promoter	140	145	.	+	0	ID=annotation1026125;Name=-10;Parent=BBa_I14016
BBa_J06148	.	engineered_region	1	151	.	+	0	ID=annotation1017859;Name=R0079;Parent=BBa_I14016
BBa_J06148	.	ribosome_entry_site	965	979	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J06148	.	engineered_region	965	979	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J06148	.	ribosome_entry_site	972	976	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J06148	.	ribosome_entry_site	965	979	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J06148	.	terminator	1691	1731	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06148	.	engineered_region	1691	1731	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06148	.	stop_codon	1724	1724	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06148	.	stem_loop	1698	1717	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06148	.	polyA_site	1718	1731	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_J06148
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacactacctct
ggcggtgatatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgccca
tcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacgg
caagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctac
ggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacg
tccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccct
ggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactac
aacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcg
aggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaa
ccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtg
accgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtag
cttaataatactagagattaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaa
ctgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaa
tggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcgg
ttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagct
ccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgact
gcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgt
tggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgt
gctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctgg
ctgtttcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata