##gff-version 3
##sequence-region BBa_J06200 1 1969
BBa_J06200	.	promoter	1	35	.	+	0	ID=BBa_I14018;Name=BBa_I14018
BBa_J06200	.	promoter	25	30	.	+	0	ID=annotation1027016;Name=-10;Parent=BBa_I14018
BBa_J06200	.	promoter	4	9	.	+	0	ID=annotation1027020;Name=-35;Parent=BBa_I14018
BBa_J06200	.	operator	1	35	.	+	0	ID=annotation1018027;Name=P(Bla);Parent=BBa_I14018
BBa_J06200	.	ribosome_entry_site	1053	1064	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06200	.	conserved_region	1057	1060	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J06200	.	CDS	62	823	.	+	0	ID=BBa_C0071;Name=BBa_C0071
BBa_J06200	.	CDS	62	784	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_J06200	.	tag	785	817	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_J06200	.	engineered_tag	824	848	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_J06200	.	terminator	1841	1920	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06200	.	stem_loop	1852	1895	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06200	.	engineered_region	1841	1920	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06200	.	engineered_region	1071	1832	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J06200	.	CDS	1071	1832	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J06200	.	stop_codon	1827	1832	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J06200	.	sequence_alteration	1312	1312	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J06200	.	engineered_region	1071	1832	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J06200	.	tag	1788	1826	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J06200	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06200	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J06200	.	terminator	857	936	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06200	.	stem_loop	868	911	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06200	.	engineered_region	857	936	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06200	.	terminator	1929	1969	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06200	.	engineered_region	1929	1969	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06200	.	stop_codon	1962	1962	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06200	.	polyA_site	1956	1969	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06200	.	stem_loop	1936	1955	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06200	.	terminator	945	985	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06200	.	engineered_region	945	985	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06200	.	stop_codon	978	978	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06200	.	polyA_site	972	985	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06200	.	stem_loop	952	971	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06200	.	promoter	994	1044	.	+	0	ID=BBa_I14017;Name=BBa_I14017
BBa_J06200	.	operator	994	1044	.	+	0	ID=annotation1043006;Name=modified P(Rhl);Parent=BBa_I14017
BBa_J06200	.	non_covalent_binding_site	994	1013	.	+	0	ID=annotation1043005;Name=RhlR;Parent=BBa_I14017
BBa_J06200	.	promoter	1014	1043	.	+	0	ID=annotation1043007;Name=-35;Parent=BBa_I14017
>BBa_J06200
tgtaagtttatacataggcgagtactctgttatggtactagagaaagaggagaaatactagatgaggaatgacggaggc
tttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaa
aggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccga
ggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctc
aacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctc
gcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcg
cgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccag
aagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaat
ggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaa
gaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgca
aacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgcatgaattggtaggcttacgcaagaaaat
ggtttgttatagtcgaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtg
gtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgcca
cctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccacctt
cggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaa
ggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcg
acaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagta
caactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccac
aacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgc
ccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctgga
gttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgct
ttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata