LOCUS       BBa_J06201               884 bp    DNA     linear       22-Dec-2024
DEFINITION  Tet repressible rhI generator (LVA+)
ACCESSION   BBa_J06201
VERSION     BBa_J06201.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..683
                     /label=rhlL
     misc_feature    684..716
                     /label=LVA
     misc_feature    723..747
                     /label=Help:Barcodes
     CDS             81..722
                     /label=BBa_C0070
     stem_loop       767..810
                     /label=stem_loop
     misc_feature    756..835
                     /label=BBa_B0010
     terminator      756..835
                     /label=BBa_B0010
     stem_loop       851..870
                     /label=T7 TE
     misc_feature    877..877
                     /label=stop
     polyA_site      871..884
                     /label=polya
     misc_feature    844..884
                     /label=BBa_B0012
     terminator      844..884
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcgaac tgctgtccga atccctggaa ggtctgtccg
      121 ctgctatgat cgctgaactg ggtcgttacc gtcaccaggt tttcatcgaa aaactgggtt
      181 gggacgttgt ttccacctcc cgtgttcgtg accaggagtt cgaccagttc gaccacccgc
      241 agacccgtta catcgttgct atgtcccgtc agggtatctg cggttgcgct cgtctgctgc
      301 cgaccaccga cgcttacctg ctgaaagacg ttttcgctta cctgtgctcc gaaaccccgc
      361 cgtccgaccc gtccgtttgg gaactgtccc gttacgctgc ttccgctgct gacgacccgc
      421 agctggctat gaaaatcttc tggtcctccc tccagtgcgc ttggtacctg ggtgcttcct
      481 ccgttgttgc tgttaccacc accgctatgg aacgttactt cgttcgtaac ggtgttatcc
      541 tccagcgtct gggtccgccg cagaaagtta aaggtgaaac cctggttgct atctccttcc
      601 cggcttacca ggaacgtggt ctggaaatgc tgctgcgtta ccacccggaa tggctccagg
      661 gtgttccgct gtccatggct gttgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      721 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc
      781 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      841 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//