LOCUS       BBa_J06204              1985 bp    DNA     linear       22-Dec-2024
DEFINITION  C4HSL receiver , CI repressor hybrid
ACCESSION   BBa_J06204
VERSION     BBa_J06204.1  
FEATURES             Location/Qualifiers
     misc_feature    1..35
                     /label=P(Bla)
     promoter        25..30
                     /label=-10
     promoter        4..9
                     /label=-35
     promoter        1..35
                     /label=BBa_I14018
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..784
                     /label=rhlR
     misc_feature    785..817
                     /label=LVA
     misc_feature    824..848
                     /label=Help:Barcodes
     CDS             62..823
                     /label=BBa_C0071
     stem_loop       868..911
                     /label=stem_loop
     misc_feature    857..936
                     /label=BBa_B0010
     terminator      857..936
                     /label=BBa_B0010
     stem_loop       952..971
                     /label=T7 TE
     misc_feature    978..978
                     /label=stop
     polyA_site      972..985
                     /label=polya
     misc_feature    945..985
                     /label=BBa_B0012
     terminator      945..985
                     /label=BBa_B0012
     misc_feature    994..1013
                     /label=RhlR
     misc_feature    994..1044
                     /label=modified P(Rhl)
     promoter        1014..1043
                     /label=-35
     promoter        994..1044
                     /label=BBa_I14017
     RBS             1053..1067
                     /label=RBS-1\Strong
     RBS             1060..1064
                     /label=RBS
     misc_feature    1053..1067
                     /label=BBa_B0030
     RBS             1053..1067
                     /label=BBa_B0030
     CDS             1077..1784
                     /label=cI lambda
     misc_feature    1785..1817
                     /label=LVA
     misc_feature    1824..1848
                     /label=Help:Barcodes
     CDS             1074..1823
                     /label=BBa_C0051
     stem_loop       1868..1911
                     /label=stem_loop
     misc_feature    1857..1936
                     /label=BBa_B0010
     terminator      1857..1936
                     /label=BBa_B0010
     stem_loop       1952..1971
                     /label=T7 TE
     misc_feature    1978..1978
                     /label=stop
     polyA_site      1972..1985
                     /label=polya
     misc_feature    1945..1985
                     /label=BBa_B0012
     terminator      1945..1985
                     /label=BBa_B0012
ORIGIN
        1 tgtaagttta tacataggcg agtactctgt tatggtacta gagaaagagg agaaatacta
       61 gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc
      121 gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt
      181 cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga agaccgaggt
      241 ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact acggggccgt
      301 ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct
      361 gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct gtgtcggcgc
      421 gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca
      481 gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt gcatgatcga
      541 gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca acccggtctg
      601 cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga gttccgggga
      661 aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca agaacatcca
      721 gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg cgctgggtct
      781 catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg
      841 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      961 tcgggtgggc ctttctgcgt ttatatacta gagtcctgca tgaattggta ggcttacgca
     1021 agaaaatggt ttgttatagt cgaatactag agattaaaga ggagaaatac tagatgagca
     1081 caaaaaagaa accattaaca caagagcagc ttgaggacgc acgtcgcctt aaagcaattt
     1141 atgaaaaaaa gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac aagatgggga
     1201 tggggcagtc aggcgttggt gctttattta atggcatcaa tgcattaaat gcttataacg
     1261 ccgcattgct tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct tcaatcgcca
     1321 gagaaatcta cgagatgtat gaagcggtta gtatgcagcc gtcacttaga agtgagtatg
     1381 agtaccctgt tttttctcat gttcaggcag ggatgttctc acctgagctt agaaccttta
     1441 ccaaaggtga tgcggagaga tgggtaagca caaccaaaaa agccagtgat tctgcattct
     1501 ggcttgaggt tgaaggtaat tccatgaccg caccaacagg ctccaagcca agctttcctg
     1561 acggaatgtt aattctcgtt gaccctgagc aggctgttga gccaggtgat ttctgcatag
     1621 ccagacttgg gggtgatgag tttaccttca agaaactgat cagggatagc ggtcaggtgt
     1681 ttttacaacc actaaaccca cagtacccaa tgatcccatg caatgagagt tgttccgttg
     1741 tggggaaagt tatcgctagt cagtggcctg aagagacgtt tggcgctgca aacgacgaaa
     1801 actacgcttt agtagcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag
     1861 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1921 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1981 ttata
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