BBa_I14017
1
RhI
P(Rhl)
2004-08-01T11:00:00Z
2015-08-31T04:07:37Z
Released HQ 2013
RhlR and C4HSL regulated promoter.
false
true
_4_
0
171
7
In stock
false
true
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
annotation1043007
1
-35
range1043007
1
21
50
annotation1043005
1
RhlR
range1043005
1
1
20
annotation1043006
1
modified P(Rhl)
range1043006
1
1
51
BBa_E0032
1
YFP
enhanced yellow fluorescent protein derived from A. victoria GFP
2003-01-31T12:00:00Z
2015-08-31T04:07:25Z
Modified from <bb_part>BBa_E0031</bb_part>
Released HQ 2013
Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. </P> Annotated mutation cause a Q81L mutation that appears to not be near the active site. </P>
false
false
_1_
0
24
7
In stock
false
<P> <P>BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation2159
1
2
range2159
1
757
762
annotation2161
1
A (Q->L)
range2161
1
242
242
annotation7041
1
BBa_E0032
range7041
1
1
762
annotation2156
1
YFP (LVA)
range2156
1
1
762
annotation2160
1
SsrA
range2160
1
718
756
BBa_J06216
1
BBa_J06216
2005-06-26T11:00:00Z
2015-08-31T04:08:17Z
false
false
_20_
0
370
20
Not in stock
false
false
orr
component1548525
1
BBa_B0031
component1548537
1
BBa_E0032
component1548520
1
BBa_I14017
annotation1548525
1
BBa_B0031
range1548525
1
60
73
annotation1548537
1
BBa_E0032
range1548537
1
80
841
annotation1548520
1
BBa_I14017
range1548520
1
1
51
BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23316
1
conserved
range23316
1
7
10
BBa_J06216_sequence
1
tcctgcatgaattggtaggcttacgcaagaaaatggtttgttatagtcgaatactagagtcacacaggaaacctactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
BBa_E0032_sequence
1
atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
BBa_B0031_sequence
1
tcacacaggaaacc
BBa_I14017_sequence
1
tcctgcatgaattggtaggcttacgcaagaaaatggtttgttatagtcgaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z