##gff-version 3
##sequence-region BBa_J06225 1 977
BBa_J06225	.	engineered_region	79	840	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J06225	.	stop_codon	835	840	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J06225	.	CDS	79	840	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J06225	.	engineered_region	79	840	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J06225	.	sequence_alteration	320	320	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J06225	.	tag	796	834	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J06225	.	promoter	1	51	.	+	0	ID=BBa_I14017;Name=BBa_I14017
BBa_J06225	.	promoter	21	50	.	+	0	ID=annotation1043007;Name=-35;Parent=BBa_I14017
BBa_J06225	.	operator	1	51	.	+	0	ID=annotation1043006;Name=modified P(Rhl);Parent=BBa_I14017
BBa_J06225	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation1043005;Name=RhlR;Parent=BBa_I14017
BBa_J06225	.	ribosome_entry_site	60	72	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J06225	.	ribosome_entry_site	60	72	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J06225	.	engineered_region	60	72	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J06225	.	ribosome_entry_site	66	69	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J06225	.	terminator	849	928	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06225	.	stem_loop	860	903	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06225	.	engineered_region	849	928	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06225	.	terminator	937	977	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06225	.	polyA_site	964	977	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06225	.	stop_codon	970	970	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06225	.	engineered_region	937	977	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06225	.	stem_loop	944	963	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_J06225
tcctgcatgaattggtaggcttacgcaagaaaatggtttgttatagtcgaatactagagtcacacaggaaagtactaga
tggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaa
gttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaag
ctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatga
agctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaa
ctacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaag
gaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcaga
agaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagca
gaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagac
cccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgt
acaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata