LOCUS       BBa_J06227              1670 bp    DNA     linear       24-Dec-2024
ACCESSION   BBa_J06227
VERSION     BBa_J06227.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     misc_feature    1..51
                     /label=modified P(Rhl)
     promoter        21..50
                     /label=-35
     promoter        1..51
                     /label=BBa_I14017
     misc_feature    64..67
                     /label=conserved
     RBS             60..71
                     /label=BBa_B0034
     CDS             78..839
                     /label=YFP (LVA)
     misc_feature    834..839
                     /label=2
     misc_feature    795..833
                     /label=SsrA
     misc_feature    319..319
                     /label=A (Q->L)
     misc_feature    78..839
                     /label=BBa_E0032
     misc_feature    78..839
                     /label=BBa_E0032
     RBS             848..860
                     /label=RBS-3\Weak
     RBS             854..857
                     /label=RBS
     misc_feature    848..860
                     /label=BBa_B0032
     RBS             848..860
                     /label=BBa_B0032
     CDS             867..1469
                     /label=rhlL
     misc_feature    1470..1502
                     /label=LVA
     misc_feature    1509..1533
                     /label=Help:Barcodes
     CDS             867..1508
                     /label=BBa_C0070
     stem_loop       1553..1596
                     /label=stem_loop
     misc_feature    1542..1621
                     /label=BBa_B0010
     terminator      1542..1621
                     /label=BBa_B0010
     stem_loop       1637..1656
                     /label=T7 TE
     misc_feature    1663..1663
                     /label=stop
     polyA_site      1657..1670
                     /label=polya
     misc_feature    1630..1670
                     /label=BBa_B0012
     terminator      1630..1670
                     /label=BBa_B0012
ORIGIN
        1 tcctgcatga attggtaggc ttacgcaaga aaatggtttg ttatagtcga atactagaga
       61 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      121 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      181 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      241 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct
      301 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      361 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      421 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      481 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      541 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      601 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      661 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
      721 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      781 acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat
      841 actagagtca cacaggaaag tactagatga tcgaactgct gtccgaatcc ctggaaggtc
      901 tgtccgctgc tatgatcgct gaactgggtc gttaccgtca ccaggttttc atcgaaaaac
      961 tgggttggga cgttgtttcc acctcccgtg ttcgtgacca ggagttcgac cagttcgacc
     1021 acccgcagac ccgttacatc gttgctatgt cccgtcaggg tatctgcggt tgcgctcgtc
     1081 tgctgccgac caccgacgct tacctgctga aagacgtttt cgcttacctg tgctccgaaa
     1141 ccccgccgtc cgacccgtcc gtttgggaac tgtcccgtta cgctgcttcc gctgctgacg
     1201 acccgcagct ggctatgaaa atcttctggt cctccctcca gtgcgcttgg tacctgggtg
     1261 cttcctccgt tgttgctgtt accaccaccg ctatggaacg ttacttcgtt cgtaacggtg
     1321 ttatcctcca gcgtctgggt ccgccgcaga aagttaaagg tgaaaccctg gttgctatct
     1381 ccttcccggc ttaccaggaa cgtggtctgg aaatgctgct gcgttaccac ccggaatggc
     1441 tccagggtgt tccgctgtcc atggctgttg ctgcaaacga cgaaaactac gctttagtag
     1501 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1561 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1621 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//