##gff-version 3
##sequence-region BBa_J06227 1 1670
BBa_J06227	.	promoter	1	51	.	+	0	ID=BBa_I14017;Name=BBa_I14017
BBa_J06227	.	promoter	21	50	.	+	0	ID=annotation1043007;Name=-35;Parent=BBa_I14017
BBa_J06227	.	operator	1	51	.	+	0	ID=annotation1043006;Name=modified P(Rhl);Parent=BBa_I14017
BBa_J06227	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation1043005;Name=RhlR;Parent=BBa_I14017
BBa_J06227	.	ribosome_entry_site	60	71	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06227	.	conserved_region	64	67	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J06227	.	ribosome_entry_site	848	860	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J06227	.	ribosome_entry_site	854	857	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J06227	.	engineered_region	848	860	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J06227	.	ribosome_entry_site	848	860	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J06227	.	CDS	867	1508	.	+	0	ID=BBa_C0070;Name=BBa_C0070
BBa_J06227	.	CDS	867	1469	.	+	0	ID=annotation301203;Name=rhlL;Parent=BBa_C0070
BBa_J06227	.	tag	1470	1502	.	+	0	ID=annotation306820;Name=LVA;Parent=BBa_C0070
BBa_J06227	.	engineered_tag	1509	1533	.	+	0	ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070
BBa_J06227	.	terminator	1630	1670	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06227	.	engineered_region	1630	1670	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06227	.	stop_codon	1663	1663	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06227	.	stem_loop	1637	1656	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06227	.	polyA_site	1657	1670	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06227	.	terminator	1542	1621	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06227	.	stem_loop	1553	1596	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06227	.	engineered_region	1542	1621	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06227	.	engineered_region	78	839	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J06227	.	CDS	78	839	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J06227	.	tag	795	833	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J06227	.	engineered_region	78	839	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J06227	.	stop_codon	834	839	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J06227	.	sequence_alteration	319	319	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
>BBa_J06227
tcctgcatgaattggtaggcttacgcaagaaaatggtttgttatagtcgaatactagagaaagaggagaaatactagat
ggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaag
ttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagc
tgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaa
gctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaac
tacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaagg
aggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaa
gaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcag
aacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagacc
ccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgta
caagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagtcacacaggaaagtactagatg
atcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttt
tcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgca
gacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctg
ctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctg
cttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttc
ctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccg
ccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgc
gttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttagtagc
ttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttata