LOCUS BBa_J06234 1981 bp DNA linear 22-Nov-2024 ACCESSION BBa_J06234 VERSION BBa_J06234.1 FEATURES Location/Qualifiers misc_feature 1..35 /label=P(Bla) promoter 25..30 /label=-10 promoter 4..9 /label=-35 promoter 1..35 /label=BBa_I14018 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..784 /label=rhlR misc_feature 785..817 /label=LVA misc_feature 824..848 /label=Help:Barcodes CDS 62..823 /label=BBa_C0071 stem_loop 868..911 /label=stem_loop misc_feature 857..936 /label=BBa_B0010 terminator 857..936 /label=BBa_B0010 stem_loop 952..971 /label=T7 TE misc_feature 978..978 /label=stop polyA_site 972..985 /label=polya misc_feature 945..985 /label=BBa_B0012 terminator 945..985 /label=BBa_B0012 misc_feature 994..1013 /label=RhlR misc_feature 994..1044 /label=modified P(Rhl) promoter 1014..1043 /label=-35 promoter 994..1044 /label=BBa_I14017 RBS 1053..1063 /label=RBS-4\Weaker RBS 1059..1062 /label=RBS misc_feature 1053..1063 /label=BBa_B0033 RBS 1053..1063 /label=BBa_B0033 CDS 1073..1780 /label=cI lambda misc_feature 1781..1813 /label=LVA misc_feature 1820..1844 /label=Help:Barcodes CDS 1070..1819 /label=BBa_C0051 stem_loop 1864..1907 /label=stem_loop misc_feature 1853..1932 /label=BBa_B0010 terminator 1853..1932 /label=BBa_B0010 stem_loop 1948..1967 /label=T7 TE misc_feature 1974..1974 /label=stop polyA_site 1968..1981 /label=polya misc_feature 1941..1981 /label=BBa_B0012 terminator 1941..1981 /label=BBa_B0012 ORIGIN 1 tgtaagttta tacataggcg agtactctgt tatggtacta gagaaagagg agaaatacta 61 gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc 121 gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt 181 cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga agaccgaggt 241 ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact acggggccgt 301 ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct 361 gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct gtgtcggcgc 421 gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca 481 gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt gcatgatcga 541 gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca acccggtctg 601 cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga gttccgggga 661 aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca agaacatcca 721 gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg cgctgggtct 781 catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg 841 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 901 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 961 tcgggtgggc ctttctgcgt ttatatacta gagtcctgca tgaattggta ggcttacgca 1021 agaaaatggt ttgttatagt cgaatactag agtcacacag gactactaga tgagcacaaa 1081 aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag caatttatga 1141 aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga tggggatggg 1201 gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt ataacgccgc 1261 attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa tcgccagaga 1321 aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg agtatgagta 1381 ccctgttttt tctcatgttc aggcagggat gttctcacct gagcttagaa cctttaccaa 1441 aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg cattctggct 1501 tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc aagccaagct ttcctgacgg 1561 aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct gcatagccag 1621 acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc aggtgttttt 1681 acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt ccgttgtggg 1741 gaaagttatc gctagtcagt ggcctgaaga gacgtttggc gctgcaaacg acgaaaacta 1801 cgctttagta gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat 1861 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1921 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 1981 a //