##gff-version 3
##sequence-region BBa_J06235 1 975
BBa_J06235	.	ribosome_entry_site	60	70	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_J06235	.	engineered_region	60	70	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_J06235	.	ribosome_entry_site	60	70	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_J06235	.	ribosome_entry_site	66	69	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_J06235	.	terminator	935	975	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06235	.	polyA_site	962	975	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06235	.	stop_codon	968	968	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06235	.	engineered_region	935	975	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06235	.	stem_loop	942	961	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06235	.	engineered_region	77	838	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J06235	.	stop_codon	833	838	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J06235	.	CDS	77	838	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J06235	.	engineered_region	77	838	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J06235	.	sequence_alteration	318	318	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J06235	.	tag	794	832	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J06235	.	promoter	1	51	.	+	0	ID=BBa_I14017;Name=BBa_I14017
BBa_J06235	.	promoter	21	50	.	+	0	ID=annotation1043007;Name=-35;Parent=BBa_I14017
BBa_J06235	.	operator	1	51	.	+	0	ID=annotation1043006;Name=modified P(Rhl);Parent=BBa_I14017
BBa_J06235	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation1043005;Name=RhlR;Parent=BBa_I14017
BBa_J06235	.	terminator	847	926	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06235	.	stem_loop	858	901	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06235	.	engineered_region	847	926	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J06235
tcctgcatgaattggtaggcttacgcaagaaaatggtttgttatagtcgaatactagagtcacacaggactactagatg
gtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagt
tcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagct
gcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaag
ctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaact
acaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaagga
ggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaag
aacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcaga
acacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccc
caacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtac
aagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttata