##gff-version 3
##sequence-region BBa_J06238 1 1610
BBa_J06238	.	terminator	1482	1561	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06238	.	stem_loop	1493	1536	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06238	.	engineered_region	1482	1561	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06238	.	terminator	1570	1610	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06238	.	polyA_site	1597	1610	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06238	.	engineered_region	1570	1610	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06238	.	stem_loop	1577	1596	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06238	.	stop_codon	1603	1603	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06238	.	ribosome_entry_site	60	71	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06238	.	conserved_region	64	67	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J06238	.	engineered_region	78	839	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J06238	.	stop_codon	834	839	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J06238	.	CDS	78	839	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J06238	.	sequence_alteration	319	319	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J06238	.	engineered_region	78	839	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J06238	.	tag	795	833	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J06238	.	CDS	865	1473	.	+	0	ID=BBa_C0170;Name=BBa_C0170
BBa_J06238	.	CDS	865	1473	.	+	0	ID=annotation1891586;Name=rhII;Parent=BBa_C0170
BBa_J06238	.	ribosome_entry_site	848	858	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_J06238	.	ribosome_entry_site	848	858	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_J06238	.	engineered_region	848	858	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_J06238	.	ribosome_entry_site	854	857	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_J06238	.	promoter	1	51	.	+	0	ID=BBa_I14017;Name=BBa_I14017
BBa_J06238	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation1043005;Name=RhlR;Parent=BBa_I14017
BBa_J06238	.	promoter	21	50	.	+	0	ID=annotation1043007;Name=-35;Parent=BBa_I14017
BBa_J06238	.	operator	1	51	.	+	0	ID=annotation1043006;Name=modified P(Rhl);Parent=BBa_I14017
>BBa_J06238
tcctgcatgaattggtaggcttacgcaagaaaatggtttgttatagtcgaatactagagaaagaggagaaatactagat
ggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaag
ttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagc
tgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaa
gctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaac
tacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaagg
aggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaa
gaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcag
aacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagacc
ccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgta
caagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagtcacacaggactactagatgat
cgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttttc
atcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcaga
cccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgct
gaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctgct
tccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttcct
ccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccgcc
gcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgcgt
taccacccggaatggctccagggtgttccgctgtccatggctgtttaataatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata