##gff-version 3
##sequence-region BBa_J06245 1 979
BBa_J06245	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06245	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06245	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06245	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06245	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06245	.	ribosome_entry_site	60	74	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J06245	.	ribosome_entry_site	67	71	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J06245	.	engineered_region	60	74	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J06245	.	ribosome_entry_site	60	74	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J06245	.	promoter	1	51	.	+	0	ID=BBa_I14017;Name=BBa_I14017
BBa_J06245	.	promoter	21	50	.	+	0	ID=annotation1043007;Name=-35;Parent=BBa_I14017
BBa_J06245	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation1043005;Name=RhlR;Parent=BBa_I14017
BBa_J06245	.	operator	1	51	.	+	0	ID=annotation1043006;Name=modified P(Rhl);Parent=BBa_I14017
BBa_J06245	.	engineered_region	81	842	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J06245	.	stop_codon	837	842	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J06245	.	tag	798	836	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J06245	.	engineered_region	81	842	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J06245	.	sequence_alteration	322	322	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J06245	.	CDS	81	842	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J06245	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06245	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06245	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J06245
tcctgcatgaattggtaggcttacgcaagaaaatggtttgttatagtcgaatactagagattaaagaggagaaatacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacat
gaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagca
gaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata