LOCUS       BBa_J06247              1672 bp    DNA     linear       24-Dec-2024
ACCESSION   BBa_J06247
VERSION     BBa_J06247.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     misc_feature    1..51
                     /label=modified P(Rhl)
     promoter        21..50
                     /label=-35
     promoter        1..51
                     /label=BBa_I14017
     misc_feature    64..67
                     /label=conserved
     RBS             60..71
                     /label=BBa_B0034
     CDS             78..839
                     /label=YFP (LVA)
     misc_feature    834..839
                     /label=2
     misc_feature    795..833
                     /label=SsrA
     misc_feature    319..319
                     /label=A (Q->L)
     misc_feature    78..839
                     /label=BBa_E0032
     misc_feature    78..839
                     /label=BBa_E0032
     RBS             848..862
                     /label=RBS-1\Strong
     RBS             855..859
                     /label=RBS
     misc_feature    848..862
                     /label=BBa_B0030
     RBS             848..862
                     /label=BBa_B0030
     CDS             869..1471
                     /label=rhlL
     misc_feature    1472..1504
                     /label=LVA
     misc_feature    1511..1535
                     /label=Help:Barcodes
     CDS             869..1510
                     /label=BBa_C0070
     stem_loop       1555..1598
                     /label=stem_loop
     misc_feature    1544..1623
                     /label=BBa_B0010
     terminator      1544..1623
                     /label=BBa_B0010
     stem_loop       1639..1658
                     /label=T7 TE
     misc_feature    1665..1665
                     /label=stop
     polyA_site      1659..1672
                     /label=polya
     misc_feature    1632..1672
                     /label=BBa_B0012
     terminator      1632..1672
                     /label=BBa_B0012
ORIGIN
        1 tcctgcatga attggtaggc ttacgcaaga aaatggtttg ttatagtcga atactagaga
       61 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      121 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      181 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      241 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct
      301 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      361 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      421 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      481 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      541 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      601 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      661 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
      721 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      781 acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat
      841 actagagatt aaagaggaga aatactagat gatcgaactg ctgtccgaat ccctggaagg
      901 tctgtccgct gctatgatcg ctgaactggg tcgttaccgt caccaggttt tcatcgaaaa
      961 actgggttgg gacgttgttt ccacctcccg tgttcgtgac caggagttcg accagttcga
     1021 ccacccgcag acccgttaca tcgttgctat gtcccgtcag ggtatctgcg gttgcgctcg
     1081 tctgctgccg accaccgacg cttacctgct gaaagacgtt ttcgcttacc tgtgctccga
     1141 aaccccgccg tccgacccgt ccgtttggga actgtcccgt tacgctgctt ccgctgctga
     1201 cgacccgcag ctggctatga aaatcttctg gtcctccctc cagtgcgctt ggtacctggg
     1261 tgcttcctcc gttgttgctg ttaccaccac cgctatggaa cgttacttcg ttcgtaacgg
     1321 tgttatcctc cagcgtctgg gtccgccgca gaaagttaaa ggtgaaaccc tggttgctat
     1381 ctccttcccg gcttaccagg aacgtggtct ggaaatgctg ctgcgttacc acccggaatg
     1441 gctccagggt gttccgctgt ccatggctgt tgctgcaaac gacgaaaact acgctttagt
     1501 agcttaataa ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa
     1561 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1621 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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