LOCUS       BBa_J06450              1652 bp    DNA     linear       25-Nov-2024
ACCESSION   BBa_J06450
VERSION     BBa_J06450.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     CDS             124..846
                     /label=BBa_E0030
     misc_feature    859..862
                     /label=conserved
     RBS             855..866
                     /label=BBa_B0034
     misc_feature    1452..1483
                     /label=LVA
     CDS             873..1451
                     /label=luxI
     misc_feature    873..1490
                     /label=BBa_C0061
     misc_feature    1491..1515
                     /label=Help:Barcodes
     CDS             873..1490
                     /label=BBa_C0061
     stem_loop       1535..1578
                     /label=stem_loop
     misc_feature    1524..1603
                     /label=BBa_B0010
     terminator      1524..1603
                     /label=BBa_B0010
     stem_loop       1619..1638
                     /label=T7 TE
     misc_feature    1645..1645
                     /label=stop
     polyA_site      1639..1652
                     /label=polya
     misc_feature    1612..1652
                     /label=BBa_B0012
     terminator      1612..1652
                     /label=BBa_B0012
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      181 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      241 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      301 accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc cgaccacatg
      361 aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      421 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      481 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      541 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
      601 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      661 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      721 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
      781 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      841 taataatact agagaaagag gagaaatact agatgactat aatgataaaa aaatcggatt
      901 ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta
      961 agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata
     1021 actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc
     1081 gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc
     1141 aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa
     1201 atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat
     1261 ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag
     1321 agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg
     1381 tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag
     1441 cagtcttaaa tgctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt
     1501 gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1561 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1621 ctcaccttcg ggtgggcctt tctgcgttta ta
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