LOCUS       BBa_J06486               897 bp    DNA     linear       23-Dec-2024
DEFINITION  B0015.E0032
ACCESSION   BBa_J06486
VERSION     BBa_J06486.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     CDS             136..897
                     /label=YFP (LVA)
     misc_feature    892..897
                     /label=2
     misc_feature    853..891
                     /label=SsrA
     misc_feature    377..377
                     /label=A (Q->L)
     misc_feature    136..897
                     /label=BBa_E0032
     misc_feature    136..897
                     /label=BBa_E0032
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      181 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      241 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      301 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac
      361 cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      421 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      481 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      541 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      601 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      661 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      721 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
      781 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      841 gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctttagtagc ttaataa
//