LOCUS       BBa_J06500              1153 bp    DNA     linear       26-Dec-2024
DEFINITION  LacI repressor (temperature-sensitive mut 241) (+LVA)
ACCESSION   BBa_J06500
VERSION     BBa_J06500.1  
FEATURES             Location/Qualifiers
     CDS             1..1128
                     /label=LacI(ts)-LVA
     misc_feature    1090..1128
                     /label=LVA
     misc_feature    1129..1153
                     /label=barcode from LacI (BBa_C0012)
     misc_feature    730..730
                     /label=TS mutation (G->A)
ORIGIN
        1 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
       61 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
      121 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
      181 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
      241 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
      301 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
      361 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
      421 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
      481 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
      541 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
      601 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
      661 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
      721 gttcccacta cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
      781 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
      841 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
      901 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
      961 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     1021 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1081 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc
     1141 tagtgtagat ctc
//