LOCUS       BBa_J06622              1928 bp    DNA     linear       10-May-2024
DEFINITION  Construction intermediate: Lambda cI QPI with Venus YFP 
            (Q04510.J06700)
ACCESSION   BBa_J06622
VERSION     BBa_J06622.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..729
                     /label=cI lambda
     misc_feature    730..762
                     /label=LVA
     misc_feature    769..793
                     /label=Help:Barcodes
     CDS             19..768
                     /label=BBa_C0051
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     promoter        976..981
                     /label=-10
     promoter        953..958
                     /label=-35
     misc_feature    963..979
                     /label=OR1
     misc_feature    939..955
                     /label=OR2
     misc_feature    939..987
                     /label=BBa_R0051
     promoter        939..987
                     /label=BBa_R0051
     misc_feature    1000..1003
                     /label=conserved
     RBS             996..1007
                     /label=BBa_B0034
     CDS             1014..1760
                     /label=Venus YFP
     misc_feature    1014..1028
                     /label=MATSG linker
     misc_feature    1746..1760
                     /label=GSGTA linker
     misc_feature    1767..1791
                     /label=Help:Barcodes
     misc_feature    1014..1766
                     /label=BBa_E2030
     stem_loop       1811..1854
                     /label=stem_loop
     misc_feature    1800..1879
                     /label=BBa_B0010
     terminator      1800..1879
                     /label=BBa_B0010
     stem_loop       1895..1914
                     /label=T7 TE
     misc_feature    1921..1921
                     /label=stop
     polyA_site      1915..1928
                     /label=polya
     misc_feature    1888..1928
                     /label=BBa_B0012
     terminator      1888..1928
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
       61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
      241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
      481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
      541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
      601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
      661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
      721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc
      781 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata tactagagta acaccgtgcg tgttgactat
      961 tttacctctg gcggtgataa tggttgctac tagagaaaga ggagaaatac tagatggcaa
     1021 ctagcggcat ggttagtaaa ggagaagaac ttttcactgg agttgtccca attttagttg
     1081 aactagatgg cgacgtgaac ggtcataagt tcagtgtctc cggcgaaggt gagggtgatg
     1141 caacgtacgg taagttaact ttgaagttaa tatgtacaac cggcaagctg cctgttccct
     1201 ggcctaccct ggtgacaacg ttaggttatg ggttgatgtg ctttgctaga tacccagatc
     1261 acatgaaaag gcatgacttc tttaaatctg caatgccaga aggttacgtc caagaacgta
     1321 ctattttctt taaagatgac ggtaattata aaactagggc tgaagttaaa ttcgaaggtg
     1381 acacacttgt aaatcgaata gagttaaagg ggattgattt caaagaggat ggtaatattc
     1441 taggccataa acttgaatat aactataatt cacacaacgt ttacattacc gccgacaagc
     1501 agaagaatgg aatcaaagcc aattttaaga ttagacacaa tattgaggat ggtggagtac
     1561 agcttgctga tcattaccaa caaaataccc cgatcggtga tggaccagtt ttgctacccg
     1621 ataaccatta tctgtcctat caaagcaaat tgtcaaaaga tcctaacgaa aaaagagacc
     1681 acatggtact cttggaattt gtaacagctg ctgggattac acatggcatg gatgaactat
     1741 acaaaggttc tggtaccgca taataaccct gatagtgcta gtgtagatcc ctactagagc
     1801 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1861 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1921 cgtttata
//