BBa_J06622
type: ComponentDefinition
persistent identity: BBa J06622
display id: BBa_J06622
version: 1
was generated by: iGEM to SBOL conversion
description: Construction intermediate: Lambda cI QPI with Venus YFP (Q04510.J06700)
created: 2005-07-05T11:00:00Z
modified: 2015-08-31T04:08:18Z
owned by: james
owned by: myers
mutable description: Combines BBa_Q04510 QPI (Lambda cI) with BBa_J06700 Reporter (Venus YFP). This is an intermediate that currently does not lead to another part.
discontinued: true
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: ytwang
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_R0051
component: BBa_B0012
component: BBa_B0010
component: BBa_C0051
component: BBa_E2030
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0051
sequence annotation: BBa_E2030
sequence: https://synbiohub.org/public/igem/BBa_J06622_sequence/1

BBa_B0034
type: Component
persistent identity: component1567067
display id: component1567067
version: 1
top level: BBa_J06622
definition: BBa_B0034
access: public

BBa_R0051
type: Component
persistent identity: component1567108
display id: component1567108
version: 1
top level: BBa_J06622
definition: cI lam
access: public

BBa_B0012
type: Component
persistent identity: component1567093
display id: component1567093
version: 1
top level: BBa_J06622
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1567083
display id: component1567083
version: 1
top level: BBa_J06622
definition: BBa_B0010
access: public

BBa_C0051
type: Component
persistent identity: component1567077
display id: component1567077
version: 1
top level: BBa_J06622
definition: cI lam
access: public

BBa_B0012
type: Component
persistent identity: component1567145
display id: component1567145
version: 1
top level: BBa_J06622
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1567135
display id: component1567135
version: 1
top level: BBa_J06622
definition: BBa_B0010
access: public

BBa_E2030
type: Component
persistent identity: component1567129
display id: component1567129
version: 1
top level: BBa_J06622
definition: BBa_E2030
access: public

BBa_B0034
type: Component
persistent identity: component1567116
display id: component1567116
version: 1
top level: BBa_J06622
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1567067
display id: annotation1567067
version: 1
top level: BBa_J06622
location: https://synbiohub.org/public/igem/BBa_J06622/annotation1567067/range1567067/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06622/annotation1567067/range1567067/1
type: Range
persistent identity: range1567067
display id: range1567067
version: 1
top level: BBa_J06622
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1567077
display id: annotation1567077
version: 1
top level: BBa_J06622
location: https://synbiohub.org/public/igem/BBa_J06622/annotation1567077/range1567077/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_J06622/annotation1567077/range1567077/1
type: Range
persistent identity: range1567077
display id: range1567077
version: 1
top level: BBa_J06622
direction: direction/forward
start: 19
end: 768
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1567083
display id: annotation1567083
version: 1
top level: BBa_J06622
location: https://synbiohub.org/public/igem/BBa_J06622/annotation1567083/range1567083/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06622/annotation1567083/range1567083/1
type: Range
persistent identity: range1567083
display id: range1567083
version: 1
top level: BBa_J06622
direction: direction/forward
start: 802
end: 881
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1567093
display id: annotation1567093
version: 1
top level: BBa_J06622
location: https://synbiohub.org/public/igem/BBa_J06622/annotation1567093/range1567093/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06622/annotation1567093/range1567093/1
type: Range
persistent identity: range1567093
display id: range1567093
version: 1
top level: BBa_J06622
direction: direction/forward
start: 890
end: 930
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1567108
display id: annotation1567108
version: 1
top level: BBa_J06622
location: https://synbiohub.org/public/igem/BBa_J06622/annotation1567108/range1567108/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J06622/annotation1567108/range1567108/1
type: Range
persistent identity: range1567108
display id: range1567108
version: 1
top level: BBa_J06622
direction: direction/forward
start: 939
end: 987
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1567116
display id: annotation1567116
version: 1
top level: BBa_J06622
location: https://synbiohub.org/public/igem/BBa_J06622/annotation1567116/range1567116/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06622/annotation1567116/range1567116/1
type: Range
persistent identity: range1567116
display id: range1567116
version: 1
top level: BBa_J06622
direction: direction/forward
start: 996
end: 1007
orientation: inline

BBa_E2030
type: SequenceAnnotation
persistent identity: annotation1567129
display id: annotation1567129
version: 1
top level: BBa_J06622
location: https://synbiohub.org/public/igem/BBa_J06622/annotation1567129/range1567129/1
component: BBa_E2030

https://synbiohub.org/public/igem/BBa_J06622/annotation1567129/range1567129/1
type: Range
persistent identity: range1567129
display id: range1567129
version: 1
top level: BBa_J06622
direction: direction/forward
start: 1014
end: 1766
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1567135
display id: annotation1567135
version: 1
top level: BBa_J06622
location: https://synbiohub.org/public/igem/BBa_J06622/annotation1567135/range1567135/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06622/annotation1567135/range1567135/1
type: Range
persistent identity: range1567135
display id: range1567135
version: 1
top level: BBa_J06622
direction: direction/forward
start: 1800
end: 1879
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1567145
display id: annotation1567145
version: 1
top level: BBa_J06622
location: https://synbiohub.org/public/igem/BBa_J06622/annotation1567145/range1567145/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06622/annotation1567145/range1567145/1
type: Range
persistent identity: range1567145
display id: range1567145
version: 1
top level: BBa_J06622
direction: direction/forward
start: 1888
end: 1928
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

BBa_E2030
type: ComponentDefinition
persistent identity: BBa E2030
display id: BBa_E2030
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced version of EYFP, yeast-optimized
created: 2005-01-24T12:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: nagai T. et al. (2002) Nat Biot (20):87-90
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Venus YFP, yeast codon optimized. EX515/EM528
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2050 (mOrange), and BBa_E2020 (Cerulean CFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Inserts a "V" after normal start M. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. Except for 5' and 3' ends no significant sequence identity runs with Cerulean CFP (BBa_E2020) or to GFP S65T as found in O'Shea deletion strain collection (originally from plasmid pFA6-GFP(S65T)-His3MX6).
star: true
creator: ryu
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Venus YFP
sequence annotation: MATSG linker
sequence annotation: GSGTA linker
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E2030_sequence/1

Venus YFP
type: SequenceAnnotation
persistent identity: annotation1431978
display id: annotation1431978
version: 1
top level: BBa_E2030
location: https://synbiohub.org/public/igem/BBa_E2030/annotation1431978/range1431978/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E2030/annotation1431978/range1431978/1
type: Range
persistent identity: range1431978
display id: range1431978
version: 1
top level: BBa_E2030
direction: direction/forward
start: 1
end: 747
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431979
display id: annotation1431979
version: 1
top level: BBa_E2030
location: https://synbiohub.org/public/igem/BBa_E2030/annotation1431979/range1431979/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_E2030/annotation1431979/range1431979/1
type: Range
persistent identity: range1431979
display id: range1431979
version: 1
top level: BBa_E2030
direction: direction/forward
start: 1
end: 15
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431980
display id: annotation1431980
version: 1
top level: BBa_E2030
location: https://synbiohub.org/public/igem/BBa_E2030/annotation1431980/range1431980/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_E2030/annotation1431980/range1431980/1
type: Range
persistent identity: range1431980
display id: range1431980
version: 1
top level: BBa_E2030
direction: direction/forward
start: 733
end: 747
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214039
display id: annotation2214039
version: 1
top level: BBa_E2030
location: https://synbiohub.org/public/igem/BBa_E2030/annotation2214039/range2214039/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E2030/annotation2214039/range2214039/1
type: Range
persistent identity: range2214039
display id: range2214039
version: 1
top level: BBa_E2030
direction: direction/forward
start: 754
end: 778
orientation: inline

https://synbiohub.org/public/igem/BBa_J06622_sequence/1
type: Sequence
persistent identity: BBa J06622 sequence
display id: BBa_J06622_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggcaactagcggcatggttagtaaaggagaagaacttttcactggagttgtcccaattttagttgaactagatggcgacgtgaacggtcataagttcagtgtctccggcgaaggtgagggtgatgcaacgtacggtaagttaactttgaagttaatatgtacaaccggcaagctgcctgttccctggcctaccctggtgacaacgttaggttatgggttgatgtgctttgctagatacccagatcacatgaaaaggcatgacttctttaaatctgcaatgccagaaggttacgtccaagaacgtactattttctttaaagatgacggtaattataaaactagggctgaagttaaattcgaaggtgacacacttgtaaatcgaatagagttaaaggggattgatttcaaagaggatggtaatattctaggccataaacttgaatataactataattcacacaacgtttacattaccgccgacaagcagaagaatggaatcaaagccaattttaagattagacacaatattgaggatggtggagtacagcttgctgatcattaccaacaaaataccccgatcggtgatggaccagttttgctacccgataaccattatctgtcctatcaaagcaaattgtcaaaagatcctaacgaaaaaagagaccacatggtactcttggaatttgtaacagctgctgggattacacatggcatggatgaactatacaaaggttctggtaccgcataataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E2030_sequence/1
type: Sequence
persistent identity: BBa E2030 sequence
display id: BBa_E2030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcaactagcggcatggttagtaaaggagaagaacttttcactggagttgtcccaattttagttgaactagatggcgacgtgaacggtcataagttcagtgtctccggcgaaggtgagggtgatgcaacgtacggtaagttaactttgaagttaatatgtacaaccggcaagctgcctgttccctggcctaccctggtgacaacgttaggttatgggttgatgtgctttgctagatacccagatcacatgaaaaggcatgacttctttaaatctgcaatgccagaaggttacgtccaagaacgtactattttctttaaagatgacggtaattataaaactagggctgaagttaaattcgaaggtgacacacttgtaaatcgaatagagttaaaggggattgatttcaaagaggatggtaatattctaggccataaacttgaatataactataattcacacaacgtttacattaccgccgacaagcagaagaatggaatcaaagccaattttaagattagacacaatattgaggatggtggagtacagcttgctgatcattaccaacaaaataccccgatcggtgatggaccagttttgctacccgataaccattatctgtcctatcaaagcaaattgtcaaaagatcctaacgaaaaaagagaccacatggtactcttggaatttgtaacagctgctgggattacacatggcatggatgaactatacaaaggttctggtaccgcataataaccctgatagtgctagtgtagatccc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z