LOCUS       BBa_J06624              1919 bp    DNA     linear       27-Dec-2024
DEFINITION  Construction intermediate: Lambda cI QPI with mCherry 
            (Q04510.J06701)
ACCESSION   BBa_J06624
VERSION     BBa_J06624.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..729
                     /label=cI lambda
     misc_feature    730..762
                     /label=LVA
     misc_feature    769..793
                     /label=Help:Barcodes
     CDS             19..768
                     /label=BBa_C0051
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     promoter        976..981
                     /label=-10
     promoter        953..958
                     /label=-35
     misc_feature    963..979
                     /label=OR1
     misc_feature    939..955
                     /label=OR2
     misc_feature    939..987
                     /label=BBa_R0051
     promoter        939..987
                     /label=BBa_R0051
     misc_feature    1000..1003
                     /label=conserved
     RBS             996..1007
                     /label=BBa_B0034
     misc_feature    1014..1028
                     /label=MATSG linker
     misc_feature    1737..1751
                     /label=GSGTA linker
     CDS             1014..1751
                     /label=mCherry
     misc_feature    1758..1782
                     /label=Help:Barcodes
     misc_feature    1014..1757
                     /label=BBa_E2060
     stem_loop       1802..1845
                     /label=stem_loop
     misc_feature    1791..1870
                     /label=BBa_B0010
     terminator      1791..1870
                     /label=BBa_B0010
     stem_loop       1886..1905
                     /label=T7 TE
     misc_feature    1912..1912
                     /label=stop
     polyA_site      1906..1919
                     /label=polya
     misc_feature    1879..1919
                     /label=BBa_B0012
     terminator      1879..1919
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
       61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
      241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
      481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
      541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
      601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
      661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
      721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc
      781 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata tactagagta acaccgtgcg tgttgactat
      961 tttacctctg gcggtgataa tggttgctac tagagaaaga ggagaaatac tagatggcaa
     1021 ctagcggcat ggttagtaaa ggagaagaaa ataacatggc aatcattaag gagttcatga
     1081 gattcaaagt tcacatggaa ggttctgtaa atggacatga atttgaaata gaaggtgaag
     1141 gagaaggaag gccttatgaa ggaacccaaa ccgcgaagct aaaagttact aagggtggcc
     1201 cattaccatt tgcatgggat atccttagcc ctcaattcat gtatgggtca aaggcttatg
     1261 tcaagcaccc cgccgacatt ccagactatc taaagttatc ttttcccgaa gggtttaagt
     1321 gggagcgtgt gatgaacttc gaagacggtg gcgtggtaac agtgactcag gattcgtccc
     1381 tgcaagatgg tgaatttatc tacaaagtca aattaagagg aactaacttt ccatctgacg
     1441 gcccggttat gcaaaaaaag acaatgggct gggaggcctc ctcagaacga atgtaccctg
     1501 aagatggtgc cttgaagggt gagattaaac aaagattgaa attgaaagat ggtggacatt
     1561 atgacgctga ggttaaaacg acatacaaag ctaagaaacc tgtccagctc ccaggtgctt
     1621 acaatgtaaa tataaaactt gatattacat cacataatga agattatacg atagttgaac
     1681 aatacgaaag ggctgagggg agacatagta ctggtggcat ggatgaacta tacaaaggtt
     1741 ctggtaccgc ataataacac tgatagtgct agtgtagatc actactagag ccaggcatca
     1801 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1861 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//