LOCUS       BBa_J06625              1835 bp    DNA     linear       14-May-2024
DEFINITION  Construction intermediate: 434 cI QPI with mCherry (Q04520.J06701)
ACCESSION   BBa_J06625
VERSION     BBa_J06625.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..687
                     /label=cI 434
     misc_feature    649..687
                     /label=LVA
     misc_feature    19..687
                     /label=BBa_C0052
     misc_feature    688..712
                     /label=Help:Barcodes
     CDS             19..687
                     /label=BBa_C0052
     stem_loop       732..775
                     /label=stem_loop
     misc_feature    721..800
                     /label=BBa_B0010
     terminator      721..800
                     /label=BBa_B0010
     stem_loop       816..835
                     /label=T7 TE
     misc_feature    842..842
                     /label=stop
     polyA_site      836..849
                     /label=polya
     misc_feature    809..849
                     /label=BBa_B0012
     terminator      809..849
                     /label=BBa_B0012
     misc_feature    858..900
                     /label=OR2
     misc_feature    865..878
                     /label=OR2
     misc_feature    887..900
                     /label=OR1
     promoter        858..863
                     /label=-35
     promoter        881..886
                     /label=-10
     promoter        859..864
                     /label=-35
     misc_feature    892..894
                     /label=start
     misc_feature    858..903
                     /label=BBa_R0052
     promoter        858..903
                     /label=BBa_R0052
     misc_feature    916..919
                     /label=conserved
     RBS             912..923
                     /label=BBa_B0034
     misc_feature    930..944
                     /label=MATSG linker
     misc_feature    1653..1667
                     /label=GSGTA linker
     CDS             930..1667
                     /label=mCherry
     misc_feature    1674..1698
                     /label=Help:Barcodes
     misc_feature    930..1673
                     /label=BBa_E2060
     stem_loop       1718..1761
                     /label=stem_loop
     misc_feature    1707..1786
                     /label=BBa_B0010
     terminator      1707..1786
                     /label=BBa_B0010
     stem_loop       1802..1821
                     /label=T7 TE
     misc_feature    1828..1828
                     /label=stop
     polyA_site      1822..1835
                     /label=polya
     misc_feature    1795..1835
                     /label=BBa_B0012
     terminator      1795..1835
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gagtatttct tccagggtaa aaagcaaaag aatccagctt
       61 ggacttaacc aggctgaact tgctcaaaag gtggggacta cccagcagtc tatagagcag
      121 ctcgaaaacg gtaaaactaa gcgaccacgc tttttaccag aacttgcgtc agctcttggc
      181 gtaagtgttg actggctgct caatggcacc tctgattcga atgttagatt tgttgggcac
      241 gttgagccca aagggaaata tccattgatt agcatggtta gagctggttc gtggtgtgaa
      301 gcttgtgaac cctacgatat caaggacatt gatgaatggt atgacagtga cgttaactta
      361 ttaggcaatg gattctggct gaaggttgaa ggtgattcca tgacctcacc tgtaggtcaa
      421 agcatccctg aaggtcatat ggtgttagta gatactggac gggagccagt gaatggaagc
      481 cttgttgtag ccaaactgac tgacgcgaac gaagcaacat tcaagaaact ggtcatagat
      541 ggcggtcaga agtacctgaa aggcctgaat ccttcatggc ctatgactcc tatcaacgga
      601 aactgcaaga ttatcggtgt tgtcgtggaa gcgagggtaa aattcgtagc tgcaaacgac
      661 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag
      721 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      781 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      841 gcgtttatat actagagttg acaaacaaga tacattgtat gaaaatacaa gaaagtttgt
      901 tgatactaga gaaagaggag aaatactaga tggcaactag cggcatggtt agtaaaggag
      961 aagaaaataa catggcaatc attaaggagt tcatgagatt caaagttcac atggaaggtt
     1021 ctgtaaatgg acatgaattt gaaatagaag gtgaaggaga aggaaggcct tatgaaggaa
     1081 cccaaaccgc gaagctaaaa gttactaagg gtggcccatt accatttgca tgggatatcc
     1141 ttagccctca attcatgtat gggtcaaagg cttatgtcaa gcaccccgcc gacattccag
     1201 actatctaaa gttatctttt cccgaagggt ttaagtggga gcgtgtgatg aacttcgaag
     1261 acggtggcgt ggtaacagtg actcaggatt cgtccctgca agatggtgaa tttatctaca
     1321 aagtcaaatt aagaggaact aactttccat ctgacggccc ggttatgcaa aaaaagacaa
     1381 tgggctggga ggcctcctca gaacgaatgt accctgaaga tggtgccttg aagggtgaga
     1441 ttaaacaaag attgaaattg aaagatggtg gacattatga cgctgaggtt aaaacgacat
     1501 acaaagctaa gaaacctgtc cagctcccag gtgcttacaa tgtaaatata aaacttgata
     1561 ttacatcaca taatgaagat tatacgatag ttgaacaata cgaaagggct gaggggagac
     1621 atagtactgg tggcatggat gaactataca aaggttctgg taccgcataa taacactgat
     1681 agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1741 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1801 tggctcacct tcgggtgggc ctttctgcgt ttata
//