BBa_J06625
type: ComponentDefinition
persistent identity: BBa J06625
display id: BBa_J06625
version: 1
was generated by: iGEM to SBOL conversion
description: Construction intermediate: 434 cI QPI with mCherry (Q04520.J06701)
created: 2005-07-05T11:00:00Z
modified: 2015-08-31T04:08:18Z
owned by: james
owned by: myers
mutable description: Combines BBa_Q04520 QPI (434 cI) with BBa_J06701 Reporter (mCherry). This is an intermediate leading to BBa_J06907, BBa_J06953, BBa_J06954, and BBa_J06955.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We have some doubts about the integrity of J06701 (mCherry with RBS and T).
star: false
creator: ytwang
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_E2060
component: BBa_B0034
component: BBa_R0052
component: BBa_C0052
sequence annotation: BBa_B0034
sequence annotation: BBa_C0052
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0052
sequence annotation: BBa_E2060
sequence: https://synbiohub.org/public/igem/BBa_J06625_sequence/1

BBa_B0012
type: Component
persistent identity: component1567432
display id: component1567432
version: 1
top level: BBa_J06625
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1567422
display id: component1567422
version: 1
top level: BBa_J06625
definition: BBa_B0010
access: public

BBa_E2060
type: Component
persistent identity: component1567416
display id: component1567416
version: 1
top level: BBa_J06625
definition: mCherry
access: public

BBa_B0034
type: Component
persistent identity: component1567403
display id: component1567403
version: 1
top level: BBa_J06625
definition: BBa_B0034
access: public

BBa_R0052
type: Component
persistent identity: component1567393
display id: component1567393
version: 1
top level: BBa_J06625
definition: cI 434
access: public

BBa_B0012
type: Component
persistent identity: component1567371
display id: component1567371
version: 1
top level: BBa_J06625
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1567361
display id: component1567361
version: 1
top level: BBa_J06625
definition: BBa_B0010
access: public

BBa_C0052
type: Component
persistent identity: component1567354
display id: component1567354
version: 1
top level: BBa_J06625
definition: cI 434
access: public

BBa_B0034
type: Component
persistent identity: component1567344
display id: component1567344
version: 1
top level: BBa_J06625
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1567344
display id: annotation1567344
version: 1
top level: BBa_J06625
location: https://synbiohub.org/public/igem/BBa_J06625/annotation1567344/range1567344/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06625/annotation1567344/range1567344/1
type: Range
persistent identity: range1567344
display id: range1567344
version: 1
top level: BBa_J06625
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation1567354
display id: annotation1567354
version: 1
top level: BBa_J06625
location: https://synbiohub.org/public/igem/BBa_J06625/annotation1567354/range1567354/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_J06625/annotation1567354/range1567354/1
type: Range
persistent identity: range1567354
display id: range1567354
version: 1
top level: BBa_J06625
direction: direction/forward
start: 19
end: 687
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1567361
display id: annotation1567361
version: 1
top level: BBa_J06625
location: https://synbiohub.org/public/igem/BBa_J06625/annotation1567361/range1567361/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06625/annotation1567361/range1567361/1
type: Range
persistent identity: range1567361
display id: range1567361
version: 1
top level: BBa_J06625
direction: direction/forward
start: 721
end: 800
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1567371
display id: annotation1567371
version: 1
top level: BBa_J06625
location: https://synbiohub.org/public/igem/BBa_J06625/annotation1567371/range1567371/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06625/annotation1567371/range1567371/1
type: Range
persistent identity: range1567371
display id: range1567371
version: 1
top level: BBa_J06625
direction: direction/forward
start: 809
end: 849
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation1567393
display id: annotation1567393
version: 1
top level: BBa_J06625
location: https://synbiohub.org/public/igem/BBa_J06625/annotation1567393/range1567393/1
component: BBa_R0052

https://synbiohub.org/public/igem/BBa_J06625/annotation1567393/range1567393/1
type: Range
persistent identity: range1567393
display id: range1567393
version: 1
top level: BBa_J06625
direction: direction/forward
start: 858
end: 903
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1567403
display id: annotation1567403
version: 1
top level: BBa_J06625
location: https://synbiohub.org/public/igem/BBa_J06625/annotation1567403/range1567403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06625/annotation1567403/range1567403/1
type: Range
persistent identity: range1567403
display id: range1567403
version: 1
top level: BBa_J06625
direction: direction/forward
start: 912
end: 923
orientation: inline

BBa_E2060
type: SequenceAnnotation
persistent identity: annotation1567416
display id: annotation1567416
version: 1
top level: BBa_J06625
location: https://synbiohub.org/public/igem/BBa_J06625/annotation1567416/range1567416/1
component: BBa_E2060

https://synbiohub.org/public/igem/BBa_J06625/annotation1567416/range1567416/1
type: Range
persistent identity: range1567416
display id: range1567416
version: 1
top level: BBa_J06625
direction: direction/forward
start: 930
end: 1673
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1567422
display id: annotation1567422
version: 1
top level: BBa_J06625
location: https://synbiohub.org/public/igem/BBa_J06625/annotation1567422/range1567422/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06625/annotation1567422/range1567422/1
type: Range
persistent identity: range1567422
display id: range1567422
version: 1
top level: BBa_J06625
direction: direction/forward
start: 1707
end: 1786
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1567432
display id: annotation1567432
version: 1
top level: BBa_J06625
location: https://synbiohub.org/public/igem/BBa_J06625/annotation1567432/range1567432/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06625/annotation1567432/range1567432/1
type: Range
persistent identity: range1567432
display id: range1567432
version: 1
top level: BBa_J06625
direction: direction/forward
start: 1795
end: 1835
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa E2060
display id: BBa_E2060
version: 1
was generated by: iGEM to SBOL conversion
description: derivative of mRFP1, yeast-optimized
created: 2005-01-24T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: Shaner et al, 2004. Nat Biotech (22):1567-1571
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, Ex587/Em610, yeast codon optimized
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Codon optimized for yeast. Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2050 (mOrange), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mOrange (BBa_E2050).
star: true
creator: ryu
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: mCherry
sequence annotation: MATSG linker
sequence annotation: GSGTA linker
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E2060_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1431932
display id: annotation1431932
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431932/range1431932/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E2060/annotation1431932/range1431932/1
type: Range
persistent identity: range1431932
display id: range1431932
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 738
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431927
display id: annotation1431927
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431927/range1431927/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E2060/annotation1431927/range1431927/1
type: Range
persistent identity: range1431927
display id: range1431927
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 15
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431931
display id: annotation1431931
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431931/range1431931/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E2060/annotation1431931/range1431931/1
type: Range
persistent identity: range1431931
display id: range1431931
version: 1
top level: mCherry
direction: direction/forward
start: 724
end: 738
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214037
display id: annotation2214037
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_E2060/annotation2214037/range2214037/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E2060/annotation2214037/range2214037/1
type: Range
persistent identity: range2214037
display id: range2214037
version: 1
top level: mCherry
direction: direction/forward
start: 745
end: 769
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa C0052
display id: BBa_C0052
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from phage 434 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage 434
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984) References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984)
star: true
creator: Maia Mahoney
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0052
sequence annotation: cI 434
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0052_sequence/1

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation7035
display id: annotation7035
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
type: Range
persistent identity: range7035
display id: range7035
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

cI 434
type: SequenceAnnotation
persistent identity: annotation1743
display id: annotation1743
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
type: Range
persistent identity: range1743
display id: range1743
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1745
display id: annotation1745
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
type: Range
persistent identity: range1745
display id: range1745
version: 1
top level: cI 434
direction: direction/forward
start: 631
end: 669
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213992
display id: annotation2213992
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
type: Range
persistent identity: range2213992
display id: range2213992
version: 1
top level: cI 434
direction: direction/forward
start: 670
end: 694
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa R0052
display id: BBa_R0052
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (434 cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage 434 right operator
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, <bb_part>BBa_C0052<bb_part>, binds to it. This segment contains O-R1, O-R2, P-R, and P-RM -35.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Maia Mahoney
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: OR2
sequence annotation: -35
sequence annotation: BBa_R0052
sequence annotation: -10
sequence annotation: OR1
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0052_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation2026
display id: annotation2026
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1
type: Range
persistent identity: range2026
display id: range2026
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2029
display id: annotation2029
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1
type: Range
persistent identity: range2029
display id: range2029
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation7068
display id: annotation7068
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1
type: Range
persistent identity: range7068
display id: range7068
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 46
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2031
display id: annotation2031
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1
type: Range
persistent identity: range2031
display id: range2031
version: 1
top level: cI 434
direction: direction/forward
start: 2
end: 7
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2027
display id: annotation2027
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1
type: Range
persistent identity: range2027
display id: range2027
version: 1
top level: cI 434
direction: direction/forward
start: 8
end: 21
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2030
display id: annotation2030
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1
type: Range
persistent identity: range2030
display id: range2030
version: 1
top level: cI 434
direction: direction/forward
start: 24
end: 29
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2028
display id: annotation2028
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1
type: Range
persistent identity: range2028
display id: range2028
version: 1
top level: cI 434
direction: direction/forward
start: 30
end: 43
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2032
display id: annotation2032
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1
type: Range
persistent identity: range2032
display id: range2032
version: 1
top level: cI 434
direction: direction/forward
start: 35
end: 37
orientation: inline

https://synbiohub.org/public/igem/BBa_J06625_sequence/1
type: Sequence
persistent identity: BBa J06625 sequence
display id: BBa_J06625_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacaaacaagatacattgtatgaaaatacaagaaagtttgttgatactagagaaagaggagaaatactagatggcaactagcggcatggttagtaaaggagaagaaaataacatggcaatcattaaggagttcatgagattcaaagttcacatggaaggttctgtaaatggacatgaatttgaaatagaaggtgaaggagaaggaaggccttatgaaggaacccaaaccgcgaagctaaaagttactaagggtggcccattaccatttgcatgggatatccttagccctcaattcatgtatgggtcaaaggcttatgtcaagcaccccgccgacattccagactatctaaagttatcttttcccgaagggtttaagtgggagcgtgtgatgaacttcgaagacggtggcgtggtaacagtgactcaggattcgtccctgcaagatggtgaatttatctacaaagtcaaattaagaggaactaactttccatctgacggcccggttatgcaaaaaaagacaatgggctgggaggcctcctcagaacgaatgtaccctgaagatggtgccttgaagggtgagattaaacaaagattgaaattgaaagatggtggacattatgacgctgaggttaaaacgacatacaaagctaagaaacctgtccagctcccaggtgcttacaatgtaaatataaaacttgatattacatcacataatgaagattatacgatagttgaacaatacgaaagggctgaggggagacatagtactggtggcatggatgaactatacaaaggttctggtaccgcataataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E2060_sequence/1
type: Sequence
persistent identity: BBa E2060 sequence
display id: BBa_E2060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcaactagcggcatggttagtaaaggagaagaaaataacatggcaatcattaaggagttcatgagattcaaagttcacatggaaggttctgtaaatggacatgaatttgaaatagaaggtgaaggagaaggaaggccttatgaaggaacccaaaccgcgaagctaaaagttactaagggtggcccattaccatttgcatgggatatccttagccctcaattcatgtatgggtcaaaggcttatgtcaagcaccccgccgacattccagactatctaaagttatcttttcccgaagggtttaagtgggagcgtgtgatgaacttcgaagacggtggcgtggtaacagtgactcaggattcgtccctgcaagatggtgaatttatctacaaagtcaaattaagaggaactaactttccatctgacggcccggttatgcaaaaaaagacaatgggctgggaggcctcctcagaacgaatgtaccctgaagatggtgccttgaagggtgagattaaacaaagattgaaattgaaagatggtggacattatgacgctgaggttaaaacgacatacaaagctaagaaacctgtccagctcccaggtgcttacaatgtaaatataaaacttgatattacatcacataatgaagattatacgatagttgaacaatacgaaagggctgaggggagacatagtactggtggcatggatgaactatacaaaggttctggtaccgcataataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0052_sequence/1
type: Sequence
persistent identity: BBa C0052 sequence
display id: BBa_C0052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0052_sequence/1
type: Sequence
persistent identity: BBa R0052 sequence
display id: BBa_R0052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacaaacaagatacattgtatgaaaatacaagaaagtttgttga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z