LOCUS       BBa_J06628              1873 bp    DNA     linear       25-Nov-2024
DEFINITION  Construction intermediate: Lambda cI QPI with mCherry, bact (LVA+) 
            (Q04510.J06703)
ACCESSION   BBa_J06628
VERSION     BBa_J06628.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..729
                     /label=cI lambda
     misc_feature    730..762
                     /label=LVA
     misc_feature    769..793
                     /label=Help:Barcodes
     CDS             19..768
                     /label=BBa_C0051
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     promoter        976..981
                     /label=-10
     promoter        953..958
                     /label=-35
     misc_feature    963..979
                     /label=OR1
     misc_feature    939..955
                     /label=OR2
     misc_feature    939..987
                     /label=BBa_R0051
     promoter        939..987
                     /label=BBa_R0051
     misc_feature    1000..1003
                     /label=conserved
     RBS             996..1007
                     /label=BBa_B0034
     CDS             1014..1721
                     /label=mCherry
     misc_feature    1365..1365
                     /label=C->T (removing PstI site)
     misc_feature    1722..1730
                     /label=LVA
     misc_feature    1014..1736
                     /label=BBa_J06505
     stem_loop       1756..1799
                     /label=stem_loop
     misc_feature    1745..1824
                     /label=BBa_B0010
     terminator      1745..1824
                     /label=BBa_B0010
     stem_loop       1840..1859
                     /label=T7 TE
     misc_feature    1866..1866
                     /label=stop
     polyA_site      1860..1873
                     /label=polya
     misc_feature    1833..1873
                     /label=BBa_B0012
     terminator      1833..1873
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
       61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
      241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
      481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
      541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
      601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
      661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
      721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc
      781 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata tactagagta acaccgtgcg tgttgactat
      961 tttacctctg gcggtgataa tggttgctac tagagaaaga ggagaaatac tagatggtga
     1021 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     1081 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     1141 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     1201 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     1261 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     1321 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccttgcag gacggcgagt
     1381 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     1441 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     1501 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     1561 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     1621 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     1681 agggccgcca ctccaccggc ggcatggacg agctgtacaa gttagtagct taataatact
     1741 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1801 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1861 ttctgcgttt ata
//