LOCUS       BBa_J06654               990 bp    DNA     linear       22-Dec-2024
DEFINITION  Test of BBa_R0051 with Venus YFP
ACCESSION   BBa_J06654
VERSION     BBa_J06654.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..822
                     /label=Venus YFP
     misc_feature    76..90
                     /label=MATSG linker
     misc_feature    808..822
                     /label=GSGTA linker
     misc_feature    829..853
                     /label=Help:Barcodes
     misc_feature    76..828
                     /label=BBa_E2030
     stem_loop       873..916
                     /label=stem_loop
     misc_feature    862..941
                     /label=BBa_B0010
     terminator      862..941
                     /label=BBa_B0010
     stem_loop       957..976
                     /label=T7 TE
     misc_feature    983..983
                     /label=stop
     polyA_site      977..990
                     /label=polya
     misc_feature    950..990
                     /label=BBa_B0012
     terminator      950..990
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggc aactagcggc atggttagta aaggagaaga acttttcact
      121 ggagttgtcc caattttagt tgaactagat ggcgacgtga acggtcataa gttcagtgtc
      181 tccggcgaag gtgagggtga tgcaacgtac ggtaagttaa ctttgaagtt aatatgtaca
      241 accggcaagc tgcctgttcc ctggcctacc ctggtgacaa cgttaggtta tgggttgatg
      301 tgctttgcta gatacccaga tcacatgaaa aggcatgact tctttaaatc tgcaatgcca
      361 gaaggttacg tccaagaacg tactattttc tttaaagatg acggtaatta taaaactagg
      421 gctgaagtta aattcgaagg tgacacactt gtaaatcgaa tagagttaaa ggggattgat
      481 ttcaaagagg atggtaatat tctaggccat aaacttgaat ataactataa ttcacacaac
      541 gtttacatta ccgccgacaa gcagaagaat ggaatcaaag ccaattttaa gattagacac
      601 aatattgagg atggtggagt acagcttgct gatcattacc aacaaaatac cccgatcggt
      661 gatggaccag ttttgctacc cgataaccat tatctgtcct atcaaagcaa attgtcaaaa
      721 gatcctaacg aaaaaagaga ccacatggta ctcttggaat ttgtaacagc tgctgggatt
      781 acacatggca tggatgaact atacaaaggt tctggtaccg cataataacc ctgatagtgc
      841 tagtgtagat ccctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      901 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      961 caccttcggg tgggcctttc tgcgtttata
//