LOCUS       BBa_J06655               987 bp    DNA     linear       22-Nov-2024
DEFINITION  Test of BBa_R0052 with Venus YFP
ACCESSION   BBa_J06655
VERSION     BBa_J06655.1  
FEATURES             Location/Qualifiers
     misc_feature    1..43
                     /label=OR2
     misc_feature    8..21
                     /label=OR2
     misc_feature    30..43
                     /label=OR1
     promoter        1..6
                     /label=-35
     promoter        24..29
                     /label=-10
     promoter        2..7
                     /label=-35
     misc_feature    35..37
                     /label=start
     misc_feature    1..46
                     /label=BBa_R0052
     promoter        1..46
                     /label=BBa_R0052
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             73..819
                     /label=Venus YFP
     misc_feature    73..87
                     /label=MATSG linker
     misc_feature    805..819
                     /label=GSGTA linker
     misc_feature    826..850
                     /label=Help:Barcodes
     misc_feature    73..825
                     /label=BBa_E2030
     stem_loop       870..913
                     /label=stem_loop
     misc_feature    859..938
                     /label=BBa_B0010
     terminator      859..938
                     /label=BBa_B0010
     stem_loop       954..973
                     /label=T7 TE
     misc_feature    980..980
                     /label=stop
     polyA_site      974..987
                     /label=polya
     misc_feature    947..987
                     /label=BBa_B0012
     terminator      947..987
                     /label=BBa_B0012
ORIGIN
        1 ttgacaaaca agatacattg tatgaaaata caagaaagtt tgttgatact agagaaagag
       61 gagaaatact agatggcaac tagcggcatg gttagtaaag gagaagaact tttcactgga
      121 gttgtcccaa ttttagttga actagatggc gacgtgaacg gtcataagtt cagtgtctcc
      181 ggcgaaggtg agggtgatgc aacgtacggt aagttaactt tgaagttaat atgtacaacc
      241 ggcaagctgc ctgttccctg gcctaccctg gtgacaacgt taggttatgg gttgatgtgc
      301 tttgctagat acccagatca catgaaaagg catgacttct ttaaatctgc aatgccagaa
      361 ggttacgtcc aagaacgtac tattttcttt aaagatgacg gtaattataa aactagggct
      421 gaagttaaat tcgaaggtga cacacttgta aatcgaatag agttaaaggg gattgatttc
      481 aaagaggatg gtaatattct aggccataaa cttgaatata actataattc acacaacgtt
      541 tacattaccg ccgacaagca gaagaatgga atcaaagcca attttaagat tagacacaat
      601 attgaggatg gtggagtaca gcttgctgat cattaccaac aaaatacccc gatcggtgat
      661 ggaccagttt tgctacccga taaccattat ctgtcctatc aaagcaaatt gtcaaaagat
      721 cctaacgaaa aaagagacca catggtactc ttggaatttg taacagctgc tgggattaca
      781 catggcatgg atgaactata caaaggttct ggtaccgcat aataaccctg atagtgctag
      841 tgtagatccc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      901 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      961 cttcgggtgg gcctttctgc gtttata
//