LOCUS       BBa_J06656              1141 bp    DNA     linear       13-May-2024
DEFINITION  Test of BBa_R0010 with Venus YFP
ACCESSION   BBa_J06656
VERSION     BBa_J06656.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..973
                     /label=Venus YFP
     misc_feature    227..241
                     /label=MATSG linker
     misc_feature    959..973
                     /label=GSGTA linker
     misc_feature    980..1004
                     /label=Help:Barcodes
     misc_feature    227..979
                     /label=BBa_E2030
     stem_loop       1024..1067
                     /label=stem_loop
     misc_feature    1013..1092
                     /label=BBa_B0010
     terminator      1013..1092
                     /label=BBa_B0010
     stem_loop       1108..1127
                     /label=T7 TE
     misc_feature    1134..1134
                     /label=stop
     polyA_site      1128..1141
                     /label=polya
     misc_feature    1101..1141
                     /label=BBa_B0012
     terminator      1101..1141
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg caactagcgg
      241 catggttagt aaaggagaag aacttttcac tggagttgtc ccaattttag ttgaactaga
      301 tggcgacgtg aacggtcata agttcagtgt ctccggcgaa ggtgagggtg atgcaacgta
      361 cggtaagtta actttgaagt taatatgtac aaccggcaag ctgcctgttc cctggcctac
      421 cctggtgaca acgttaggtt atgggttgat gtgctttgct agatacccag atcacatgaa
      481 aaggcatgac ttctttaaat ctgcaatgcc agaaggttac gtccaagaac gtactatttt
      541 ctttaaagat gacggtaatt ataaaactag ggctgaagtt aaattcgaag gtgacacact
      601 tgtaaatcga atagagttaa aggggattga tttcaaagag gatggtaata ttctaggcca
      661 taaacttgaa tataactata attcacacaa cgtttacatt accgccgaca agcagaagaa
      721 tggaatcaaa gccaatttta agattagaca caatattgag gatggtggag tacagcttgc
      781 tgatcattac caacaaaata ccccgatcgg tgatggacca gttttgctac ccgataacca
      841 ttatctgtcc tatcaaagca aattgtcaaa agatcctaac gaaaaaagag accacatggt
      901 actcttggaa tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaagg
      961 ttctggtacc gcataataac cctgatagtg ctagtgtaga tccctactag agccaggcat
     1021 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1081 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1141 a
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