LOCUS       BBa_J06657               996 bp    DNA     linear       22-Dec-2024
DEFINITION  Test of BBa_R0011 with Venus YFP
ACCESSION   BBa_J06657
VERSION     BBa_J06657.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..828
                     /label=Venus YFP
     misc_feature    82..96
                     /label=MATSG linker
     misc_feature    814..828
                     /label=GSGTA linker
     misc_feature    835..859
                     /label=Help:Barcodes
     misc_feature    82..834
                     /label=BBa_E2030
     stem_loop       879..922
                     /label=stem_loop
     misc_feature    868..947
                     /label=BBa_B0010
     terminator      868..947
                     /label=BBa_B0010
     stem_loop       963..982
                     /label=T7 TE
     misc_feature    989..989
                     /label=stop
     polyA_site      983..996
                     /label=polya
     misc_feature    956..996
                     /label=BBa_B0012
     terminator      956..996
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggcaact agcggcatgg ttagtaaagg agaagaactt
      121 ttcactggag ttgtcccaat tttagttgaa ctagatggcg acgtgaacgg tcataagttc
      181 agtgtctccg gcgaaggtga gggtgatgca acgtacggta agttaacttt gaagttaata
      241 tgtacaaccg gcaagctgcc tgttccctgg cctaccctgg tgacaacgtt aggttatggg
      301 ttgatgtgct ttgctagata cccagatcac atgaaaaggc atgacttctt taaatctgca
      361 atgccagaag gttacgtcca agaacgtact attttcttta aagatgacgg taattataaa
      421 actagggctg aagttaaatt cgaaggtgac acacttgtaa atcgaataga gttaaagggg
      481 attgatttca aagaggatgg taatattcta ggccataaac ttgaatataa ctataattca
      541 cacaacgttt acattaccgc cgacaagcag aagaatggaa tcaaagccaa ttttaagatt
      601 agacacaata ttgaggatgg tggagtacag cttgctgatc attaccaaca aaataccccg
      661 atcggtgatg gaccagtttt gctacccgat aaccattatc tgtcctatca aagcaaattg
      721 tcaaaagatc ctaacgaaaa aagagaccac atggtactct tggaatttgt aacagctgct
      781 gggattacac atggcatgga tgaactatac aaaggttctg gtaccgcata ataaccctga
      841 tagtgctagt gtagatccct actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      901 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      961 ctggctcacc ttcgggtggg cctttctgcg tttata
//