LOCUS       BBa_J06659               978 bp    DNA     linear       22-Nov-2024
DEFINITION  Test of BBa_R0052 with mCherry
ACCESSION   BBa_J06659
VERSION     BBa_J06659.1  
FEATURES             Location/Qualifiers
     misc_feature    1..43
                     /label=OR2
     misc_feature    8..21
                     /label=OR2
     misc_feature    30..43
                     /label=OR1
     promoter        1..6
                     /label=-35
     promoter        24..29
                     /label=-10
     promoter        2..7
                     /label=-35
     misc_feature    35..37
                     /label=start
     misc_feature    1..46
                     /label=BBa_R0052
     promoter        1..46
                     /label=BBa_R0052
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     misc_feature    73..87
                     /label=MATSG linker
     misc_feature    796..810
                     /label=GSGTA linker
     CDS             73..810
                     /label=mCherry
     misc_feature    817..841
                     /label=Help:Barcodes
     misc_feature    73..816
                     /label=BBa_E2060
     stem_loop       861..904
                     /label=stem_loop
     misc_feature    850..929
                     /label=BBa_B0010
     terminator      850..929
                     /label=BBa_B0010
     stem_loop       945..964
                     /label=T7 TE
     misc_feature    971..971
                     /label=stop
     polyA_site      965..978
                     /label=polya
     misc_feature    938..978
                     /label=BBa_B0012
     terminator      938..978
                     /label=BBa_B0012
ORIGIN
        1 ttgacaaaca agatacattg tatgaaaata caagaaagtt tgttgatact agagaaagag
       61 gagaaatact agatggcaac tagcggcatg gttagtaaag gagaagaaaa taacatggca
      121 atcattaagg agttcatgag attcaaagtt cacatggaag gttctgtaaa tggacatgaa
      181 tttgaaatag aaggtgaagg agaaggaagg ccttatgaag gaacccaaac cgcgaagcta
      241 aaagttacta agggtggccc attaccattt gcatgggata tccttagccc tcaattcatg
      301 tatgggtcaa aggcttatgt caagcacccc gccgacattc cagactatct aaagttatct
      361 tttcccgaag ggtttaagtg ggagcgtgtg atgaacttcg aagacggtgg cgtggtaaca
      421 gtgactcagg attcgtccct gcaagatggt gaatttatct acaaagtcaa attaagagga
      481 actaactttc catctgacgg cccggttatg caaaaaaaga caatgggctg ggaggcctcc
      541 tcagaacgaa tgtaccctga agatggtgcc ttgaagggtg agattaaaca aagattgaaa
      601 ttgaaagatg gtggacatta tgacgctgag gttaaaacga catacaaagc taagaaacct
      661 gtccagctcc caggtgctta caatgtaaat ataaaacttg atattacatc acataatgaa
      721 gattatacga tagttgaaca atacgaaagg gctgagggga gacatagtac tggtggcatg
      781 gatgaactat acaaaggttc tggtaccgca taataacact gatagtgcta gtgtagatca
      841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      961 ggcctttctg cgtttata
//