LOCUS       BBa_J06660              1132 bp    DNA     linear       14-May-2024
DEFINITION  Test of BBa_R0010 with mCherry
ACCESSION   BBa_J06660
VERSION     BBa_J06660.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..241
                     /label=MATSG linker
     misc_feature    950..964
                     /label=GSGTA linker
     CDS             227..964
                     /label=mCherry
     misc_feature    971..995
                     /label=Help:Barcodes
     misc_feature    227..970
                     /label=BBa_E2060
     stem_loop       1015..1058
                     /label=stem_loop
     misc_feature    1004..1083
                     /label=BBa_B0010
     terminator      1004..1083
                     /label=BBa_B0010
     stem_loop       1099..1118
                     /label=T7 TE
     misc_feature    1125..1125
                     /label=stop
     polyA_site      1119..1132
                     /label=polya
     misc_feature    1092..1132
                     /label=BBa_B0012
     terminator      1092..1132
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg caactagcgg
      241 catggttagt aaaggagaag aaaataacat ggcaatcatt aaggagttca tgagattcaa
      301 agttcacatg gaaggttctg taaatggaca tgaatttgaa atagaaggtg aaggagaagg
      361 aaggccttat gaaggaaccc aaaccgcgaa gctaaaagtt actaagggtg gcccattacc
      421 atttgcatgg gatatcctta gccctcaatt catgtatggg tcaaaggctt atgtcaagca
      481 ccccgccgac attccagact atctaaagtt atcttttccc gaagggttta agtgggagcg
      541 tgtgatgaac ttcgaagacg gtggcgtggt aacagtgact caggattcgt ccctgcaaga
      601 tggtgaattt atctacaaag tcaaattaag aggaactaac tttccatctg acggcccggt
      661 tatgcaaaaa aagacaatgg gctgggaggc ctcctcagaa cgaatgtacc ctgaagatgg
      721 tgccttgaag ggtgagatta aacaaagatt gaaattgaaa gatggtggac attatgacgc
      781 tgaggttaaa acgacataca aagctaagaa acctgtccag ctcccaggtg cttacaatgt
      841 aaatataaaa cttgatatta catcacataa tgaagattat acgatagttg aacaatacga
      901 aagggctgag gggagacata gtactggtgg catggatgaa ctatacaaag gttctggtac
      961 cgcataataa cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa
     1021 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1081 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//