LOCUS       BBa_J06661               987 bp    DNA     linear       14-May-2024
DEFINITION  Test of BBa_R0011 with mCherry
ACCESSION   BBa_J06661
VERSION     BBa_J06661.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..96
                     /label=MATSG linker
     misc_feature    805..819
                     /label=GSGTA linker
     CDS             82..819
                     /label=mCherry
     misc_feature    826..850
                     /label=Help:Barcodes
     misc_feature    82..825
                     /label=BBa_E2060
     stem_loop       870..913
                     /label=stem_loop
     misc_feature    859..938
                     /label=BBa_B0010
     terminator      859..938
                     /label=BBa_B0010
     stem_loop       954..973
                     /label=T7 TE
     misc_feature    980..980
                     /label=stop
     polyA_site      974..987
                     /label=polya
     misc_feature    947..987
                     /label=BBa_B0012
     terminator      947..987
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggcaact agcggcatgg ttagtaaagg agaagaaaat
      121 aacatggcaa tcattaagga gttcatgaga ttcaaagttc acatggaagg ttctgtaaat
      181 ggacatgaat ttgaaataga aggtgaagga gaaggaaggc cttatgaagg aacccaaacc
      241 gcgaagctaa aagttactaa gggtggccca ttaccatttg catgggatat ccttagccct
      301 caattcatgt atgggtcaaa ggcttatgtc aagcaccccg ccgacattcc agactatcta
      361 aagttatctt ttcccgaagg gtttaagtgg gagcgtgtga tgaacttcga agacggtggc
      421 gtggtaacag tgactcagga ttcgtccctg caagatggtg aatttatcta caaagtcaaa
      481 ttaagaggaa ctaactttcc atctgacggc ccggttatgc aaaaaaagac aatgggctgg
      541 gaggcctcct cagaacgaat gtaccctgaa gatggtgcct tgaagggtga gattaaacaa
      601 agattgaaat tgaaagatgg tggacattat gacgctgagg ttaaaacgac atacaaagct
      661 aagaaacctg tccagctccc aggtgcttac aatgtaaata taaaacttga tattacatca
      721 cataatgaag attatacgat agttgaacaa tacgaaaggg ctgaggggag acatagtact
      781 ggtggcatgg atgaactata caaaggttct ggtaccgcat aataacactg atagtgctag
      841 tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      901 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      961 cttcgggtgg gcctttctgc gtttata
//