LOCUS       BBa_J06703               878 bp    DNA     linear       22-Nov-2024
DEFINITION  mCherry, bacterial (LVA+) with RBS and forward terminator
ACCESSION   BBa_J06703
VERSION     BBa_J06703.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..726
                     /label=mCherry
     misc_feature    370..370
                     /label=C->T (removing PstI site)
     misc_feature    727..735
                     /label=LVA
     misc_feature    19..741
                     /label=BBa_J06505
     stem_loop       761..804
                     /label=stem_loop
     misc_feature    750..829
                     /label=BBa_B0010
     terminator      750..829
                     /label=BBa_B0010
     stem_loop       845..864
                     /label=T7 TE
     misc_feature    871..871
                     /label=stop
     polyA_site      865..878
                     /label=polya
     misc_feature    838..878
                     /label=BBa_B0012
     terminator      838..878
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
       61 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      121 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      181 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      241 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      301 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      361 gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      421 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
      481 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
      541 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      601 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      661 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      721 tacaagttag tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      781 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      841 cactggctca ccttcgggtg ggcctttctg cgtttata
//