##gff-version 3
##sequence-region BBa_J06800 1 1370
BBa_J06800	.	terminator	1180	1259	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J06800	.	engineered_region	1180	1259	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J06800	.	stem_loop	1191	1234	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J06800	.	promoter	1317	1370	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J06800	.	promoter	1359	1364	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J06800	.	engineered_region	1317	1370	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J06800	.	operator	1319	1335	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J06800	.	promoter	1336	1341	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J06800	.	operator	1342	1358	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J06800	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J06800	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J06800	.	terminator	1268	1308	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J06800	.	stop_codon	1301	1301	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J06800	.	polyA_site	1295	1308	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J06800	.	stem_loop	1275	1294	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J06800	.	engineered_region	1268	1308	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J06800	.	CDS	19	1171	.	+	0	ID=BBa_J06500;Name=BBa_J06500
BBa_J06800	.	sequence_alteration	748	748	.	+	0	ID=annotation1518230;Name=TS mutation (G->A);Parent=BBa_J06500
BBa_J06800	.	engineered_tag	1147	1171	.	+	0	ID=annotation1518229;Name=barcode from LacI (BBa_C0012);Parent=BBa_J06500
BBa_J06800	.	tag	1108	1146	.	+	0	ID=annotation1518220;Name=LVA;Parent=BBa_J06500
BBa_J06800	.	CDS	19	1146	.	+	0	ID=annotation1518213;Name=LacI(ts)-LVA;Parent=BBa_J06500
>BBa_J06800
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactacgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacatt
gtgagcggataacaagatactgagcaca