LOCUS       BBa_J06901              2360 bp    DNA     linear       22-Nov-2024
DEFINITION  LacI ts (mut 241) QPI regulated by lambda cI with mCherry
ACCESSION   BBa_J06901
VERSION     BBa_J06901.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..1203
                     /label=LacI(ts)-LVA
     misc_feature    1165..1203
                     /label=LVA
     misc_feature    1204..1228
                     /label=barcode from LacI (BBa_C0012)
     misc_feature    805..805
                     /label=TS mutation (G->A)
     CDS             76..1228
                     /label=BBa_J06500
     stem_loop       1248..1291
                     /label=stem_loop
     misc_feature    1237..1316
                     /label=BBa_B0010
     terminator      1237..1316
                     /label=BBa_B0010
     stem_loop       1332..1351
                     /label=T7 TE
     misc_feature    1358..1358
                     /label=stop
     polyA_site      1352..1365
                     /label=polya
     misc_feature    1325..1365
                     /label=BBa_B0012
     terminator      1325..1365
                     /label=BBa_B0012
     misc_feature    1376..1392
                     /label=lac O1
     promoter        1393..1398
                     /label=-35
     misc_feature    1399..1415
                     /label=lac O1
     promoter        1416..1421
                     /label=-10
     misc_feature    1374..1427
                     /label=BBa_R0011
     promoter        1374..1427
                     /label=BBa_R0011
     misc_feature    1441..1444
                     /label=conserved
     RBS             1437..1448
                     /label=BBa_B0034
     misc_feature    1455..1469
                     /label=MATSG linker
     misc_feature    2178..2192
                     /label=GSGTA linker
     CDS             1455..2192
                     /label=mCherry
     misc_feature    2199..2223
                     /label=Help:Barcodes
     misc_feature    1455..2198
                     /label=BBa_E2060
     stem_loop       2243..2286
                     /label=stem_loop
     misc_feature    2232..2311
                     /label=BBa_B0010
     terminator      2232..2311
                     /label=BBa_B0010
     stem_loop       2327..2346
                     /label=T7 TE
     misc_feature    2353..2353
                     /label=stop
     polyA_site      2347..2360
                     /label=polya
     misc_feature    2320..2360
                     /label=BBa_B0012
     terminator      2320..2360
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
      121 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
      181 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
      241 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
      301 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
      361 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
      421 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
      481 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
      541 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
      601 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
      661 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
      721 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
      781 ctgaatgagg gcatcgttcc cactacgatg ctggttgcca acgatcagat ggcgctgggc
      841 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
      901 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
      961 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     1021 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     1081 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1141 gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa
     1201 taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg
     1261 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1321 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagaattgtg
     1381 agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata ctagagaaag
     1441 aggagaaata ctagatggca actagcggca tggttagtaa aggagaagaa aataacatgg
     1501 caatcattaa ggagttcatg agattcaaag ttcacatgga aggttctgta aatggacatg
     1561 aatttgaaat agaaggtgaa ggagaaggaa ggccttatga aggaacccaa accgcgaagc
     1621 taaaagttac taagggtggc ccattaccat ttgcatggga tatccttagc cctcaattca
     1681 tgtatgggtc aaaggcttat gtcaagcacc ccgccgacat tccagactat ctaaagttat
     1741 cttttcccga agggtttaag tgggagcgtg tgatgaactt cgaagacggt ggcgtggtaa
     1801 cagtgactca ggattcgtcc ctgcaagatg gtgaatttat ctacaaagtc aaattaagag
     1861 gaactaactt tccatctgac ggcccggtta tgcaaaaaaa gacaatgggc tgggaggcct
     1921 cctcagaacg aatgtaccct gaagatggtg ccttgaaggg tgagattaaa caaagattga
     1981 aattgaaaga tggtggacat tatgacgctg aggttaaaac gacatacaaa gctaagaaac
     2041 ctgtccagct cccaggtgct tacaatgtaa atataaaact tgatattaca tcacataatg
     2101 aagattatac gatagttgaa caatacgaaa gggctgaggg gagacatagt actggtggca
     2161 tggatgaact atacaaaggt tctggtaccg cataataaca ctgatagtgc tagtgtagat
     2221 cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2281 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2341 tgggcctttc tgcgtttata
//