LOCUS       BBa_J06904              1973 bp    DNA     linear       13-May-2024
DEFINITION  Lambda cI857 (E07700) QPI regulated by 434 cI with mCherry
ACCESSION   BBa_J06904
VERSION     BBa_J06904.1  
FEATURES             Location/Qualifiers
     misc_feature    1..43
                     /label=OR2
     misc_feature    8..21
                     /label=OR2
     misc_feature    30..43
                     /label=OR1
     promoter        1..6
                     /label=-35
     promoter        24..29
                     /label=-10
     promoter        2..7
                     /label=-35
     misc_feature    35..37
                     /label=start
     misc_feature    1..46
                     /label=BBa_R0052
     promoter        1..46
                     /label=BBa_R0052
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             73..783
                     /label=cI857
     misc_feature    784..816
                     /label=LVA
     misc_feature    320..320
                     /label=G->C
     misc_feature    424..424
                     /label=G->A (ind-)
     misc_feature    753..753
                     /label=C->G
     misc_feature    823..847
                     /label=barcode from lambda cI (BBa_C0051)
     CDS             73..847
                     /label=BBa_J06502
     stem_loop       867..910
                     /label=stem_loop
     misc_feature    856..935
                     /label=BBa_B0010
     terminator      856..935
                     /label=BBa_B0010
     stem_loop       951..970
                     /label=T7 TE
     misc_feature    977..977
                     /label=stop
     polyA_site      971..984
                     /label=polya
     misc_feature    944..984
                     /label=BBa_B0012
     terminator      944..984
                     /label=BBa_B0012
     promoter        1030..1035
                     /label=-10
     promoter        1007..1012
                     /label=-35
     misc_feature    1017..1033
                     /label=OR1
     misc_feature    993..1009
                     /label=OR2
     misc_feature    993..1041
                     /label=BBa_R0051
     promoter        993..1041
                     /label=BBa_R0051
     misc_feature    1054..1057
                     /label=conserved
     RBS             1050..1061
                     /label=BBa_B0034
     misc_feature    1068..1082
                     /label=MATSG linker
     misc_feature    1791..1805
                     /label=GSGTA linker
     CDS             1068..1805
                     /label=mCherry
     misc_feature    1812..1836
                     /label=Help:Barcodes
     misc_feature    1068..1811
                     /label=BBa_E2060
     stem_loop       1856..1899
                     /label=stem_loop
     misc_feature    1845..1924
                     /label=BBa_B0010
     terminator      1845..1924
                     /label=BBa_B0010
     stem_loop       1940..1959
                     /label=T7 TE
     misc_feature    1966..1966
                     /label=stop
     polyA_site      1960..1973
                     /label=polya
     misc_feature    1933..1973
                     /label=BBa_B0012
     terminator      1933..1973
                     /label=BBa_B0012
ORIGIN
        1 ttgacaaaca agatacattg tatgaaaata caagaaagtt tgttgatact agagaaagag
       61 gagaaatact agatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca
      121 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct
      181 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat
      241 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa
      301 tttagccctt caatcgccac agaaatctac gagatgtatg aagcggttag tatgcagccg
      361 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca
      421 cctaagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa
      481 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc
      541 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag
      601 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc
      661 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc
      721 aatgagagtt gttccgttgt ggggaaagtt atggctagtc agtggcctga agagacgttt
      781 ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt
      841 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      901 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      961 cgggtgggcc tttctgcgtt tatatactag agtaacaccg tgcgtgttga ctattttacc
     1021 tctggcggtg ataatggttg ctactagaga aagaggagaa atactagatg gcaactagcg
     1081 gcatggttag taaaggagaa gaaaataaca tggcaatcat taaggagttc atgagattca
     1141 aagttcacat ggaaggttct gtaaatggac atgaatttga aatagaaggt gaaggagaag
     1201 gaaggcctta tgaaggaacc caaaccgcga agctaaaagt tactaagggt ggcccattac
     1261 catttgcatg ggatatcctt agccctcaat tcatgtatgg gtcaaaggct tatgtcaagc
     1321 accccgccga cattccagac tatctaaagt tatcttttcc cgaagggttt aagtgggagc
     1381 gtgtgatgaa cttcgaagac ggtggcgtgg taacagtgac tcaggattcg tccctgcaag
     1441 atggtgaatt tatctacaaa gtcaaattaa gaggaactaa ctttccatct gacggcccgg
     1501 ttatgcaaaa aaagacaatg ggctgggagg cctcctcaga acgaatgtac cctgaagatg
     1561 gtgccttgaa gggtgagatt aaacaaagat tgaaattgaa agatggtgga cattatgacg
     1621 ctgaggttaa aacgacatac aaagctaaga aacctgtcca gctcccaggt gcttacaatg
     1681 taaatataaa acttgatatt acatcacata atgaagatta tacgatagtt gaacaatacg
     1741 aaagggctga ggggagacat agtactggtg gcatggatga actatacaaa ggttctggta
     1801 ccgcataata acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa
     1861 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1921 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//