BBa_J06904
type: ComponentDefinition
persistent identity: BBa J06904
display id: BBa_J06904
version: 1
was generated by: iGEM to SBOL conversion
description: Lambda cI857 (E07700) QPI regulated by 434 cI with mCherry
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: true
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: ytwang
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_R0051
component: BBa_E2060
component: BBa_B0034
component: BBa_R0052
component: BBa_J06502
sequence annotation: BBa_R0052
sequence annotation: BBa_B0034
sequence annotation: BBa_J06502
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0051
sequence annotation: BBa_E2060
sequence: https://synbiohub.org/public/igem/BBa_J06904_sequence/1

BBa_B0012
type: Component
persistent identity: component1564046
display id: component1564046
version: 1
top level: BBa_J06904
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1564036
display id: component1564036
version: 1
top level: BBa_J06904
definition: BBa_B0010
access: public

BBa_R0051
type: Component
persistent identity: component1564009
display id: component1564009
version: 1
top level: BBa_J06904
definition: cI lam
access: public

BBa_B0012
type: Component
persistent identity: component1563994
display id: component1563994
version: 1
top level: BBa_J06904
definition: BBa_B0012
access: public

BBa_E2060
type: Component
persistent identity: component1564030
display id: component1564030
version: 1
top level: BBa_J06904
definition: mCherry
access: public

BBa_B0034
type: Component
persistent identity: component1564017
display id: component1564017
version: 1
top level: BBa_J06904
definition: BBa_B0034
access: public

BBa_B0034
type: Component
persistent identity: component1563959
display id: component1563959
version: 1
top level: BBa_J06904
definition: BBa_B0034
access: public

BBa_R0052
type: Component
persistent identity: component1563949
display id: component1563949
version: 1
top level: BBa_J06904
definition: cI 434
access: public

BBa_B0010
type: Component
persistent identity: component1563984
display id: component1563984
version: 1
top level: BBa_J06904
definition: BBa_B0010
access: public

BBa_J06502
type: Component
persistent identity: component1563979
display id: component1563979
version: 1
top level: BBa_J06904
definition: cI ts
access: public

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation1563949
display id: annotation1563949
version: 1
top level: BBa_J06904
location: https://synbiohub.org/public/igem/BBa_J06904/annotation1563949/range1563949/1
component: BBa_R0052

https://synbiohub.org/public/igem/BBa_J06904/annotation1563949/range1563949/1
type: Range
persistent identity: range1563949
display id: range1563949
version: 1
top level: BBa_J06904
direction: direction/forward
start: 1
end: 46
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1563959
display id: annotation1563959
version: 1
top level: BBa_J06904
location: https://synbiohub.org/public/igem/BBa_J06904/annotation1563959/range1563959/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06904/annotation1563959/range1563959/1
type: Range
persistent identity: range1563959
display id: range1563959
version: 1
top level: BBa_J06904
direction: direction/forward
start: 55
end: 66
orientation: inline

BBa_J06502
type: SequenceAnnotation
persistent identity: annotation1563979
display id: annotation1563979
version: 1
top level: BBa_J06904
location: https://synbiohub.org/public/igem/BBa_J06904/annotation1563979/range1563979/1
component: BBa_J06502

https://synbiohub.org/public/igem/BBa_J06904/annotation1563979/range1563979/1
type: Range
persistent identity: range1563979
display id: range1563979
version: 1
top level: BBa_J06904
direction: direction/forward
start: 73
end: 847
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1563984
display id: annotation1563984
version: 1
top level: BBa_J06904
location: https://synbiohub.org/public/igem/BBa_J06904/annotation1563984/range1563984/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06904/annotation1563984/range1563984/1
type: Range
persistent identity: range1563984
display id: range1563984
version: 1
top level: BBa_J06904
direction: direction/forward
start: 856
end: 935
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1563994
display id: annotation1563994
version: 1
top level: BBa_J06904
location: https://synbiohub.org/public/igem/BBa_J06904/annotation1563994/range1563994/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06904/annotation1563994/range1563994/1
type: Range
persistent identity: range1563994
display id: range1563994
version: 1
top level: BBa_J06904
direction: direction/forward
start: 944
end: 984
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1564009
display id: annotation1564009
version: 1
top level: BBa_J06904
location: https://synbiohub.org/public/igem/BBa_J06904/annotation1564009/range1564009/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J06904/annotation1564009/range1564009/1
type: Range
persistent identity: range1564009
display id: range1564009
version: 1
top level: BBa_J06904
direction: direction/forward
start: 993
end: 1041
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1564017
display id: annotation1564017
version: 1
top level: BBa_J06904
location: https://synbiohub.org/public/igem/BBa_J06904/annotation1564017/range1564017/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06904/annotation1564017/range1564017/1
type: Range
persistent identity: range1564017
display id: range1564017
version: 1
top level: BBa_J06904
direction: direction/forward
start: 1050
end: 1061
orientation: inline

BBa_E2060
type: SequenceAnnotation
persistent identity: annotation1564030
display id: annotation1564030
version: 1
top level: BBa_J06904
location: https://synbiohub.org/public/igem/BBa_J06904/annotation1564030/range1564030/1
component: BBa_E2060

https://synbiohub.org/public/igem/BBa_J06904/annotation1564030/range1564030/1
type: Range
persistent identity: range1564030
display id: range1564030
version: 1
top level: BBa_J06904
direction: direction/forward
start: 1068
end: 1811
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1564036
display id: annotation1564036
version: 1
top level: BBa_J06904
location: https://synbiohub.org/public/igem/BBa_J06904/annotation1564036/range1564036/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06904/annotation1564036/range1564036/1
type: Range
persistent identity: range1564036
display id: range1564036
version: 1
top level: BBa_J06904
direction: direction/forward
start: 1845
end: 1924
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1564046
display id: annotation1564046
version: 1
top level: BBa_J06904
location: https://synbiohub.org/public/igem/BBa_J06904/annotation1564046/range1564046/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06904/annotation1564046/range1564046/1
type: Range
persistent identity: range1564046
display id: range1564046
version: 1
top level: BBa_J06904
direction: direction/forward
start: 1933
end: 1973
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa E2060
display id: BBa_E2060
version: 1
was generated by: iGEM to SBOL conversion
description: derivative of mRFP1, yeast-optimized
created: 2005-01-24T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: Shaner et al, 2004. Nat Biotech (22):1567-1571
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, Ex587/Em610, yeast codon optimized
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Codon optimized for yeast. Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2050 (mOrange), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mOrange (BBa_E2050).
star: true
creator: ryu
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: mCherry
sequence annotation: MATSG linker
sequence annotation: GSGTA linker
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E2060_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1431932
display id: annotation1431932
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431932/range1431932/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E2060/annotation1431932/range1431932/1
type: Range
persistent identity: range1431932
display id: range1431932
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 738
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431927
display id: annotation1431927
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431927/range1431927/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E2060/annotation1431927/range1431927/1
type: Range
persistent identity: range1431927
display id: range1431927
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 15
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431931
display id: annotation1431931
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431931/range1431931/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E2060/annotation1431931/range1431931/1
type: Range
persistent identity: range1431931
display id: range1431931
version: 1
top level: mCherry
direction: direction/forward
start: 724
end: 738
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214037
display id: annotation2214037
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_E2060/annotation2214037/range2214037/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E2060/annotation2214037/range2214037/1
type: Range
persistent identity: range2214037
display id: range2214037
version: 1
top level: mCherry
direction: direction/forward
start: 745
end: 769
orientation: inline

cI ts
type: ComponentDefinition
persistent identity: BBa J06502
display id: BBa_J06502
version: 1
was generated by: iGEM to SBOL conversion
description: Lambda cI857 (E07700) (RBS- LVA+)
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
mutable provenance: GenBank E00770 (Japanese Patent)
owned by: james
owned by: myers
mutable description: Mutated from BBa_C0051 Repressor, Lambda cI (RBS- LVA+) to be temperature sensitive and UV insensitive.
discontinued: true
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: First attempt to mutate failed. Suspect the problem is a low mutation rate due to results of positive control. May be a result of the mutagenesis kit being old. Second attempt to mutation failed. The best we got was 2 out of 3 mutations. The sequencing had a lot of bad sequence at the beginings, so that the ind- mutation was not sequenced by either of the primers used. The ind- mutation was tested by cutting with HindIII. This part is being put on hold. We may finish it by doing a single site mutation on one of the mutants with the other 2 sites successfully mutated.
star: false
creator: ytwang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI857
sequence annotation: G->C
sequence annotation: G->A (ind-)
sequence annotation: C->G
sequence annotation: LVA
sequence annotation: barcode from lambda cI (BBa_C0051)
sequence: https://synbiohub.org/public/igem/BBa_J06502_sequence/1

cI857
type: SequenceAnnotation
persistent identity: annotation1531454
display id: annotation1531454
version: 1
top level: cI ts
location: https://synbiohub.org/public/igem/BBa_J06502/annotation1531454/range1531454/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J06502/annotation1531454/range1531454/1
type: Range
persistent identity: range1531454
display id: range1531454
version: 1
top level: cI ts
direction: direction/forward
start: 1
end: 711
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1550568
display id: annotation1550568
version: 1
top level: cI ts
location: https://synbiohub.org/public/igem/BBa_J06502/annotation1550568/range1550568/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J06502/annotation1550568/range1550568/1
type: Range
persistent identity: range1550568
display id: range1550568
version: 1
top level: cI ts
direction: direction/forward
start: 248
end: 248
orientation: inline

G->A (ind-)
type: SequenceAnnotation
persistent identity: annotation1550569
display id: annotation1550569
version: 1
top level: cI ts
location: https://synbiohub.org/public/igem/BBa_J06502/annotation1550569/range1550569/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J06502/annotation1550569/range1550569/1
type: Range
persistent identity: range1550569
display id: range1550569
version: 1
top level: cI ts
direction: direction/forward
start: 352
end: 352
orientation: inline

C->G
type: SequenceAnnotation
persistent identity: annotation1550572
display id: annotation1550572
version: 1
top level: cI ts
location: https://synbiohub.org/public/igem/BBa_J06502/annotation1550572/range1550572/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J06502/annotation1550572/range1550572/1
type: Range
persistent identity: range1550572
display id: range1550572
version: 1
top level: cI ts
direction: direction/forward
start: 681
end: 681
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1531464
display id: annotation1531464
version: 1
top level: cI ts
location: https://synbiohub.org/public/igem/BBa_J06502/annotation1531464/range1531464/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J06502/annotation1531464/range1531464/1
type: Range
persistent identity: range1531464
display id: range1531464
version: 1
top level: cI ts
direction: direction/forward
start: 712
end: 744
orientation: inline

barcode from lambda cI (BBa_C0051)
type: SequenceAnnotation
persistent identity: annotation1552045
display id: annotation1552045
version: 1
top level: cI ts
location: https://synbiohub.org/public/igem/BBa_J06502/annotation1552045/range1552045/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_J06502/annotation1552045/range1552045/1
type: Range
persistent identity: range1552045
display id: range1552045
version: 1
top level: cI ts
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa R0052
display id: BBa_R0052
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (434 cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage 434 right operator
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, <bb_part>BBa_C0052<bb_part>, binds to it. This segment contains O-R1, O-R2, P-R, and P-RM -35.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: -35
sequence annotation: BBa_R0052
sequence annotation: -10
sequence annotation: OR1
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0052_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation2026
display id: annotation2026
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1
type: Range
persistent identity: range2026
display id: range2026
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2029
display id: annotation2029
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1
type: Range
persistent identity: range2029
display id: range2029
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation7068
display id: annotation7068
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1
type: Range
persistent identity: range7068
display id: range7068
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 46
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2031
display id: annotation2031
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1
type: Range
persistent identity: range2031
display id: range2031
version: 1
top level: cI 434
direction: direction/forward
start: 2
end: 7
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2027
display id: annotation2027
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1
type: Range
persistent identity: range2027
display id: range2027
version: 1
top level: cI 434
direction: direction/forward
start: 8
end: 21
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2030
display id: annotation2030
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1
type: Range
persistent identity: range2030
display id: range2030
version: 1
top level: cI 434
direction: direction/forward
start: 24
end: 29
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2028
display id: annotation2028
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1
type: Range
persistent identity: range2028
display id: range2028
version: 1
top level: cI 434
direction: direction/forward
start: 30
end: 43
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2032
display id: annotation2032
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1
type: Range
persistent identity: range2032
display id: range2032
version: 1
top level: cI 434
direction: direction/forward
start: 35
end: 37
orientation: inline

https://synbiohub.org/public/igem/BBa_J06904_sequence/1
type: Sequence
persistent identity: BBa J06904 sequence
display id: BBa_J06904_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacaaacaagatacattgtatgaaaatacaagaaagtttgttgatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccacagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctaagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatggctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggcaactagcggcatggttagtaaaggagaagaaaataacatggcaatcattaaggagttcatgagattcaaagttcacatggaaggttctgtaaatggacatgaatttgaaatagaaggtgaaggagaaggaaggccttatgaaggaacccaaaccgcgaagctaaaagttactaagggtggcccattaccatttgcatgggatatccttagccctcaattcatgtatgggtcaaaggcttatgtcaagcaccccgccgacattccagactatctaaagttatcttttcccgaagggtttaagtgggagcgtgtgatgaacttcgaagacggtggcgtggtaacagtgactcaggattcgtccctgcaagatggtgaatttatctacaaagtcaaattaagaggaactaactttccatctgacggcccggttatgcaaaaaaagacaatgggctgggaggcctcctcagaacgaatgtaccctgaagatggtgccttgaagggtgagattaaacaaagattgaaattgaaagatggtggacattatgacgctgaggttaaaacgacatacaaagctaagaaacctgtccagctcccaggtgcttacaatgtaaatataaaacttgatattacatcacataatgaagattatacgatagttgaacaatacgaaagggctgaggggagacatagtactggtggcatggatgaactatacaaaggttctggtaccgcataataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E2060_sequence/1
type: Sequence
persistent identity: BBa E2060 sequence
display id: BBa_E2060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcaactagcggcatggttagtaaaggagaagaaaataacatggcaatcattaaggagttcatgagattcaaagttcacatggaaggttctgtaaatggacatgaatttgaaatagaaggtgaaggagaaggaaggccttatgaaggaacccaaaccgcgaagctaaaagttactaagggtggcccattaccatttgcatgggatatccttagccctcaattcatgtatgggtcaaaggcttatgtcaagcaccccgccgacattccagactatctaaagttatcttttcccgaagggtttaagtgggagcgtgtgatgaacttcgaagacggtggcgtggtaacagtgactcaggattcgtccctgcaagatggtgaatttatctacaaagtcaaattaagaggaactaactttccatctgacggcccggttatgcaaaaaaagacaatgggctgggaggcctcctcagaacgaatgtaccctgaagatggtgccttgaagggtgagattaaacaaagattgaaattgaaagatggtggacattatgacgctgaggttaaaacgacatacaaagctaagaaacctgtccagctcccaggtgcttacaatgtaaatataaaacttgatattacatcacataatgaagattatacgatagttgaacaatacgaaagggctgaggggagacatagtactggtggcatggatgaactatacaaaggttctggtaccgcataataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06502_sequence/1
type: Sequence
persistent identity: BBa J06502 sequence
display id: BBa_J06502_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccacagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctaagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatggctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0052_sequence/1
type: Sequence
persistent identity: BBa R0052 sequence
display id: BBa_R0052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacaaacaagatacattgtatgaaaatacaagaaagtttgttga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z