LOCUS       BBa_J06906              1982 bp    DNA     linear       13-May-2024
DEFINITION  Lambda cI QPI regulated by LacI with mCherry
ACCESSION   BBa_J06906
VERSION     BBa_J06906.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..792
                     /label=cI lambda
     misc_feature    793..825
                     /label=LVA
     misc_feature    832..856
                     /label=Help:Barcodes
     CDS             82..831
                     /label=BBa_C0051
     stem_loop       876..919
                     /label=stem_loop
     misc_feature    865..944
                     /label=BBa_B0010
     terminator      865..944
                     /label=BBa_B0010
     stem_loop       960..979
                     /label=T7 TE
     misc_feature    986..986
                     /label=stop
     polyA_site      980..993
                     /label=polya
     misc_feature    953..993
                     /label=BBa_B0012
     terminator      953..993
                     /label=BBa_B0012
     promoter        1039..1044
                     /label=-10
     promoter        1016..1021
                     /label=-35
     misc_feature    1026..1042
                     /label=OR1
     misc_feature    1002..1018
                     /label=OR2
     misc_feature    1002..1050
                     /label=BBa_R0051
     promoter        1002..1050
                     /label=BBa_R0051
     misc_feature    1063..1066
                     /label=conserved
     RBS             1059..1070
                     /label=BBa_B0034
     misc_feature    1077..1091
                     /label=MATSG linker
     misc_feature    1800..1814
                     /label=GSGTA linker
     CDS             1077..1814
                     /label=mCherry
     misc_feature    1821..1845
                     /label=Help:Barcodes
     misc_feature    1077..1820
                     /label=BBa_E2060
     stem_loop       1865..1908
                     /label=stem_loop
     misc_feature    1854..1933
                     /label=BBa_B0010
     terminator      1854..1933
                     /label=BBa_B0010
     stem_loop       1949..1968
                     /label=T7 TE
     misc_feature    1975..1975
                     /label=stop
     polyA_site      1969..1982
                     /label=polya
     misc_feature    1942..1982
                     /label=BBa_B0012
     terminator      1942..1982
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt
      121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc
      181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat
      241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc
      301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt
      361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg
      421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca
      481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca
      541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag
      601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag
      661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg
      721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa
      781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag
      841 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      901 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      961 gctcaccttc gggtgggcct ttctgcgttt atatactaga gtaacaccgt gcgtgttgac
     1021 tattttacct ctggcggtga taatggttgc tactagagaa agaggagaaa tactagatgg
     1081 caactagcgg catggttagt aaaggagaag aaaataacat ggcaatcatt aaggagttca
     1141 tgagattcaa agttcacatg gaaggttctg taaatggaca tgaatttgaa atagaaggtg
     1201 aaggagaagg aaggccttat gaaggaaccc aaaccgcgaa gctaaaagtt actaagggtg
     1261 gcccattacc atttgcatgg gatatcctta gccctcaatt catgtatggg tcaaaggctt
     1321 atgtcaagca ccccgccgac attccagact atctaaagtt atcttttccc gaagggttta
     1381 agtgggagcg tgtgatgaac ttcgaagacg gtggcgtggt aacagtgact caggattcgt
     1441 ccctgcaaga tggtgaattt atctacaaag tcaaattaag aggaactaac tttccatctg
     1501 acggcccggt tatgcaaaaa aagacaatgg gctgggaggc ctcctcagaa cgaatgtacc
     1561 ctgaagatgg tgccttgaag ggtgagatta aacaaagatt gaaattgaaa gatggtggac
     1621 attatgacgc tgaggttaaa acgacataca aagctaagaa acctgtccag ctcccaggtg
     1681 cttacaatgt aaatataaaa cttgatatta catcacataa tgaagattat acgatagttg
     1741 aacaatacga aagggctgag gggagacata gtactggtgg catggatgaa ctatacaaag
     1801 gttctggtac cgcataataa cactgatagt gctagtgtag atcactacta gagccaggca
     1861 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1921 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1981 ta
//