LOCUS       BBa_J06907              1892 bp    DNA     linear       14-May-2024
DEFINITION  434 cI QPI regulated by Lambda cI with mCherry
ACCESSION   BBa_J06907
VERSION     BBa_J06907.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..744
                     /label=cI 434
     misc_feature    706..744
                     /label=LVA
     misc_feature    76..744
                     /label=BBa_C0052
     misc_feature    745..769
                     /label=Help:Barcodes
     CDS             76..744
                     /label=BBa_C0052
     stem_loop       789..832
                     /label=stem_loop
     misc_feature    778..857
                     /label=BBa_B0010
     terminator      778..857
                     /label=BBa_B0010
     stem_loop       873..892
                     /label=T7 TE
     misc_feature    899..899
                     /label=stop
     polyA_site      893..906
                     /label=polya
     misc_feature    866..906
                     /label=BBa_B0012
     terminator      866..906
                     /label=BBa_B0012
     misc_feature    915..957
                     /label=OR2
     misc_feature    922..935
                     /label=OR2
     misc_feature    944..957
                     /label=OR1
     promoter        915..920
                     /label=-35
     promoter        938..943
                     /label=-10
     promoter        916..921
                     /label=-35
     misc_feature    949..951
                     /label=start
     misc_feature    915..960
                     /label=BBa_R0052
     promoter        915..960
                     /label=BBa_R0052
     misc_feature    973..976
                     /label=conserved
     RBS             969..980
                     /label=BBa_B0034
     misc_feature    987..1001
                     /label=MATSG linker
     misc_feature    1710..1724
                     /label=GSGTA linker
     CDS             987..1724
                     /label=mCherry
     misc_feature    1731..1755
                     /label=Help:Barcodes
     misc_feature    987..1730
                     /label=BBa_E2060
     stem_loop       1775..1818
                     /label=stem_loop
     misc_feature    1764..1843
                     /label=BBa_B0010
     terminator      1764..1843
                     /label=BBa_B0010
     stem_loop       1859..1878
                     /label=T7 TE
     misc_feature    1885..1885
                     /label=stop
     polyA_site      1879..1892
                     /label=polya
     misc_feature    1852..1892
                     /label=BBa_B0012
     terminator      1852..1892
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgag tatttcttcc agggtaaaaa gcaaaagaat ccagcttgga
      121 cttaaccagg ctgaacttgc tcaaaaggtg gggactaccc agcagtctat agagcagctc
      181 gaaaacggta aaactaagcg accacgcttt ttaccagaac ttgcgtcagc tcttggcgta
      241 agtgttgact ggctgctcaa tggcacctct gattcgaatg ttagatttgt tgggcacgtt
      301 gagcccaaag ggaaatatcc attgattagc atggttagag ctggttcgtg gtgtgaagct
      361 tgtgaaccct acgatatcaa ggacattgat gaatggtatg acagtgacgt taacttatta
      421 ggcaatggat tctggctgaa ggttgaaggt gattccatga cctcacctgt aggtcaaagc
      481 atccctgaag gtcatatggt gttagtagat actggacggg agccagtgaa tggaagcctt
      541 gttgtagcca aactgactga cgcgaacgaa gcaacattca agaaactggt catagatggc
      601 ggtcagaagt acctgaaagg cctgaatcct tcatggccta tgactcctat caacggaaac
      661 tgcaagatta tcggtgttgt cgtggaagcg agggtaaaat tcgtagctgc aaacgacgaa
      721 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca
      781 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      841 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      901 tttatatact agagttgaca aacaagatac attgtatgaa aatacaagaa agtttgttga
      961 tactagagaa agaggagaaa tactagatgg caactagcgg catggttagt aaaggagaag
     1021 aaaataacat ggcaatcatt aaggagttca tgagattcaa agttcacatg gaaggttctg
     1081 taaatggaca tgaatttgaa atagaaggtg aaggagaagg aaggccttat gaaggaaccc
     1141 aaaccgcgaa gctaaaagtt actaagggtg gcccattacc atttgcatgg gatatcctta
     1201 gccctcaatt catgtatggg tcaaaggctt atgtcaagca ccccgccgac attccagact
     1261 atctaaagtt atcttttccc gaagggttta agtgggagcg tgtgatgaac ttcgaagacg
     1321 gtggcgtggt aacagtgact caggattcgt ccctgcaaga tggtgaattt atctacaaag
     1381 tcaaattaag aggaactaac tttccatctg acggcccggt tatgcaaaaa aagacaatgg
     1441 gctgggaggc ctcctcagaa cgaatgtacc ctgaagatgg tgccttgaag ggtgagatta
     1501 aacaaagatt gaaattgaaa gatggtggac attatgacgc tgaggttaaa acgacataca
     1561 aagctaagaa acctgtccag ctcccaggtg cttacaatgt aaatataaaa cttgatatta
     1621 catcacataa tgaagattat acgatagttg aacaatacga aagggctgag gggagacata
     1681 gtactggtgg catggatgaa ctatacaaag gttctggtac cgcataataa cactgatagt
     1741 gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1801 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1861 ctcaccttcg ggtgggcctt tctgcgttta ta
//