BBa_J06907
type: ComponentDefinition
persistent identity: BBa J06907
display id: BBa_J06907
version: 1
was generated by: iGEM to SBOL conversion
description: 434 cI QPI regulated by Lambda cI with mCherry
created: 2005-06-27T11:00:00Z
modified: 2015-08-31T04:08:19Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: true
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: ytwang
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_R0051
component: BBa_B0010
component: BBa_C0052
component: BBa_B0012
component: BBa_R0052
component: BBa_E2060
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_C0052
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0052
sequence annotation: BBa_E2060
sequence: https://synbiohub.org/public/igem/BBa_J06907_sequence/1

BBa_B0034
type: Component
persistent identity: component1564395
display id: component1564395
version: 1
top level: BBa_J06907
definition: BBa_B0034
access: public

BBa_R0051
type: Component
persistent identity: component1564387
display id: component1564387
version: 1
top level: BBa_J06907
definition: cI lam
access: public

BBa_B0010
type: Component
persistent identity: component1564412
display id: component1564412
version: 1
top level: BBa_J06907
definition: BBa_B0010
access: public

BBa_C0052
type: Component
persistent identity: component1564405
display id: component1564405
version: 1
top level: BBa_J06907
definition: cI 434
access: public

BBa_B0012
type: Component
persistent identity: component1564483
display id: component1564483
version: 1
top level: BBa_J06907
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1564473
display id: component1564473
version: 1
top level: BBa_J06907
definition: BBa_B0010
access: public

BBa_R0052
type: Component
persistent identity: component1564444
display id: component1564444
version: 1
top level: BBa_J06907
definition: cI 434
access: public

BBa_B0012
type: Component
persistent identity: component1564422
display id: component1564422
version: 1
top level: BBa_J06907
definition: BBa_B0012
access: public

BBa_E2060
type: Component
persistent identity: component1564467
display id: component1564467
version: 1
top level: BBa_J06907
definition: mCherry
access: public

BBa_B0034
type: Component
persistent identity: component1564454
display id: component1564454
version: 1
top level: BBa_J06907
definition: BBa_B0034
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1564387
display id: annotation1564387
version: 1
top level: BBa_J06907
location: https://synbiohub.org/public/igem/BBa_J06907/annotation1564387/range1564387/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J06907/annotation1564387/range1564387/1
type: Range
persistent identity: range1564387
display id: range1564387
version: 1
top level: BBa_J06907
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1564395
display id: annotation1564395
version: 1
top level: BBa_J06907
location: https://synbiohub.org/public/igem/BBa_J06907/annotation1564395/range1564395/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06907/annotation1564395/range1564395/1
type: Range
persistent identity: range1564395
display id: range1564395
version: 1
top level: BBa_J06907
direction: direction/forward
start: 58
end: 69
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation1564405
display id: annotation1564405
version: 1
top level: BBa_J06907
location: https://synbiohub.org/public/igem/BBa_J06907/annotation1564405/range1564405/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_J06907/annotation1564405/range1564405/1
type: Range
persistent identity: range1564405
display id: range1564405
version: 1
top level: BBa_J06907
direction: direction/forward
start: 76
end: 744
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1564412
display id: annotation1564412
version: 1
top level: BBa_J06907
location: https://synbiohub.org/public/igem/BBa_J06907/annotation1564412/range1564412/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06907/annotation1564412/range1564412/1
type: Range
persistent identity: range1564412
display id: range1564412
version: 1
top level: BBa_J06907
direction: direction/forward
start: 778
end: 857
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1564422
display id: annotation1564422
version: 1
top level: BBa_J06907
location: https://synbiohub.org/public/igem/BBa_J06907/annotation1564422/range1564422/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06907/annotation1564422/range1564422/1
type: Range
persistent identity: range1564422
display id: range1564422
version: 1
top level: BBa_J06907
direction: direction/forward
start: 866
end: 906
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation1564444
display id: annotation1564444
version: 1
top level: BBa_J06907
location: https://synbiohub.org/public/igem/BBa_J06907/annotation1564444/range1564444/1
component: BBa_R0052

https://synbiohub.org/public/igem/BBa_J06907/annotation1564444/range1564444/1
type: Range
persistent identity: range1564444
display id: range1564444
version: 1
top level: BBa_J06907
direction: direction/forward
start: 915
end: 960
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1564454
display id: annotation1564454
version: 1
top level: BBa_J06907
location: https://synbiohub.org/public/igem/BBa_J06907/annotation1564454/range1564454/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06907/annotation1564454/range1564454/1
type: Range
persistent identity: range1564454
display id: range1564454
version: 1
top level: BBa_J06907
direction: direction/forward
start: 969
end: 980
orientation: inline

BBa_E2060
type: SequenceAnnotation
persistent identity: annotation1564467
display id: annotation1564467
version: 1
top level: BBa_J06907
location: https://synbiohub.org/public/igem/BBa_J06907/annotation1564467/range1564467/1
component: BBa_E2060

https://synbiohub.org/public/igem/BBa_J06907/annotation1564467/range1564467/1
type: Range
persistent identity: range1564467
display id: range1564467
version: 1
top level: BBa_J06907
direction: direction/forward
start: 987
end: 1730
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1564473
display id: annotation1564473
version: 1
top level: BBa_J06907
location: https://synbiohub.org/public/igem/BBa_J06907/annotation1564473/range1564473/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06907/annotation1564473/range1564473/1
type: Range
persistent identity: range1564473
display id: range1564473
version: 1
top level: BBa_J06907
direction: direction/forward
start: 1764
end: 1843
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1564483
display id: annotation1564483
version: 1
top level: BBa_J06907
location: https://synbiohub.org/public/igem/BBa_J06907/annotation1564483/range1564483/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06907/annotation1564483/range1564483/1
type: Range
persistent identity: range1564483
display id: range1564483
version: 1
top level: BBa_J06907
direction: direction/forward
start: 1852
end: 1892
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa E2060
display id: BBa_E2060
version: 1
was generated by: iGEM to SBOL conversion
description: derivative of mRFP1, yeast-optimized
created: 2005-01-24T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: Shaner et al, 2004. Nat Biotech (22):1567-1571
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, Ex587/Em610, yeast codon optimized
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Codon optimized for yeast. Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2050 (mOrange), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mOrange (BBa_E2050).
star: true
creator: ryu
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: mCherry
sequence annotation: MATSG linker
sequence annotation: GSGTA linker
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E2060_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1431932
display id: annotation1431932
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431932/range1431932/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E2060/annotation1431932/range1431932/1
type: Range
persistent identity: range1431932
display id: range1431932
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 738
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431927
display id: annotation1431927
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431927/range1431927/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E2060/annotation1431927/range1431927/1
type: Range
persistent identity: range1431927
display id: range1431927
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 15
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431931
display id: annotation1431931
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431931/range1431931/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E2060/annotation1431931/range1431931/1
type: Range
persistent identity: range1431931
display id: range1431931
version: 1
top level: mCherry
direction: direction/forward
start: 724
end: 738
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214037
display id: annotation2214037
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_E2060/annotation2214037/range2214037/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E2060/annotation2214037/range2214037/1
type: Range
persistent identity: range2214037
display id: range2214037
version: 1
top level: mCherry
direction: direction/forward
start: 745
end: 769
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa C0052
display id: BBa_C0052
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from phage 434 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage 434
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984) References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984)
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0052
sequence annotation: cI 434
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0052_sequence/1

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation7035
display id: annotation7035
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
type: Range
persistent identity: range7035
display id: range7035
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

cI 434
type: SequenceAnnotation
persistent identity: annotation1743
display id: annotation1743
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
type: Range
persistent identity: range1743
display id: range1743
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1745
display id: annotation1745
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
type: Range
persistent identity: range1745
display id: range1745
version: 1
top level: cI 434
direction: direction/forward
start: 631
end: 669
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213992
display id: annotation2213992
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
type: Range
persistent identity: range2213992
display id: range2213992
version: 1
top level: cI 434
direction: direction/forward
start: 670
end: 694
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa R0052
display id: BBa_R0052
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (434 cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage 434 right operator
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, <bb_part>BBa_C0052<bb_part>, binds to it. This segment contains O-R1, O-R2, P-R, and P-RM -35.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: -35
sequence annotation: BBa_R0052
sequence annotation: -10
sequence annotation: OR1
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0052_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation2026
display id: annotation2026
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1
type: Range
persistent identity: range2026
display id: range2026
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2029
display id: annotation2029
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1
type: Range
persistent identity: range2029
display id: range2029
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation7068
display id: annotation7068
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1
type: Range
persistent identity: range7068
display id: range7068
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 46
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2031
display id: annotation2031
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1
type: Range
persistent identity: range2031
display id: range2031
version: 1
top level: cI 434
direction: direction/forward
start: 2
end: 7
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2027
display id: annotation2027
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1
type: Range
persistent identity: range2027
display id: range2027
version: 1
top level: cI 434
direction: direction/forward
start: 8
end: 21
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2030
display id: annotation2030
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1
type: Range
persistent identity: range2030
display id: range2030
version: 1
top level: cI 434
direction: direction/forward
start: 24
end: 29
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2028
display id: annotation2028
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1
type: Range
persistent identity: range2028
display id: range2028
version: 1
top level: cI 434
direction: direction/forward
start: 30
end: 43
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2032
display id: annotation2032
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1
type: Range
persistent identity: range2032
display id: range2032
version: 1
top level: cI 434
direction: direction/forward
start: 35
end: 37
orientation: inline

https://synbiohub.org/public/igem/BBa_J06907_sequence/1
type: Sequence
persistent identity: BBa J06907 sequence
display id: BBa_J06907_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacaaacaagatacattgtatgaaaatacaagaaagtttgttgatactagagaaagaggagaaatactagatggcaactagcggcatggttagtaaaggagaagaaaataacatggcaatcattaaggagttcatgagattcaaagttcacatggaaggttctgtaaatggacatgaatttgaaatagaaggtgaaggagaaggaaggccttatgaaggaacccaaaccgcgaagctaaaagttactaagggtggcccattaccatttgcatgggatatccttagccctcaattcatgtatgggtcaaaggcttatgtcaagcaccccgccgacattccagactatctaaagttatcttttcccgaagggtttaagtgggagcgtgtgatgaacttcgaagacggtggcgtggtaacagtgactcaggattcgtccctgcaagatggtgaatttatctacaaagtcaaattaagaggaactaactttccatctgacggcccggttatgcaaaaaaagacaatgggctgggaggcctcctcagaacgaatgtaccctgaagatggtgccttgaagggtgagattaaacaaagattgaaattgaaagatggtggacattatgacgctgaggttaaaacgacatacaaagctaagaaacctgtccagctcccaggtgcttacaatgtaaatataaaacttgatattacatcacataatgaagattatacgatagttgaacaatacgaaagggctgaggggagacatagtactggtggcatggatgaactatacaaaggttctggtaccgcataataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E2060_sequence/1
type: Sequence
persistent identity: BBa E2060 sequence
display id: BBa_E2060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcaactagcggcatggttagtaaaggagaagaaaataacatggcaatcattaaggagttcatgagattcaaagttcacatggaaggttctgtaaatggacatgaatttgaaatagaaggtgaaggagaaggaaggccttatgaaggaacccaaaccgcgaagctaaaagttactaagggtggcccattaccatttgcatgggatatccttagccctcaattcatgtatgggtcaaaggcttatgtcaagcaccccgccgacattccagactatctaaagttatcttttcccgaagggtttaagtgggagcgtgtgatgaacttcgaagacggtggcgtggtaacagtgactcaggattcgtccctgcaagatggtgaatttatctacaaagtcaaattaagaggaactaactttccatctgacggcccggttatgcaaaaaaagacaatgggctgggaggcctcctcagaacgaatgtaccctgaagatggtgccttgaagggtgagattaaacaaagattgaaattgaaagatggtggacattatgacgctgaggttaaaacgacatacaaagctaagaaacctgtccagctcccaggtgcttacaatgtaaatataaaacttgatattacatcacataatgaagattatacgatagttgaacaatacgaaagggctgaggggagacatagtactggtggcatggatgaactatacaaaggttctggtaccgcataataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0052_sequence/1
type: Sequence
persistent identity: BBa C0052 sequence
display id: BBa_C0052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0052_sequence/1
type: Sequence
persistent identity: BBa R0052 sequence
display id: BBa_R0052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacaaacaagatacattgtatgaaaatacaagaaagtttgttga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z