LOCUS       BBa_J06912              2314 bp    DNA     linear       09-May-2024
DEFINITION  LacI ts (mut 265) QPI regulated by lambda cI with EYFP
ACCESSION   BBa_J06912
VERSION     BBa_J06912.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..1203
                     /label=LacI(ts)-LVA
     misc_feature    878..878
                     /label=TS mutation (G->A)
     misc_feature    1165..1203
                     /label=LVA
     misc_feature    1204..1228
                     /label=barcode from LacI (BBa_C0012)
     CDS             76..1228
                     /label=BBa_J06501
     stem_loop       1248..1291
                     /label=stem_loop
     misc_feature    1237..1316
                     /label=BBa_B0010
     terminator      1237..1316
                     /label=BBa_B0010
     stem_loop       1332..1351
                     /label=T7 TE
     misc_feature    1358..1358
                     /label=stop
     polyA_site      1352..1365
                     /label=polya
     misc_feature    1325..1365
                     /label=BBa_B0012
     terminator      1325..1365
                     /label=BBa_B0012
     misc_feature    1376..1392
                     /label=lac O1
     promoter        1393..1398
                     /label=-35
     misc_feature    1399..1415
                     /label=lac O1
     promoter        1416..1421
                     /label=-10
     misc_feature    1374..1427
                     /label=BBa_R0011
     promoter        1374..1427
                     /label=BBa_R0011
     misc_feature    1441..1444
                     /label=conserved
     RBS             1437..1448
                     /label=BBa_B0034
     CDS             1455..2177
                     /label=BBa_E0030
     stem_loop       2197..2240
                     /label=stem_loop
     misc_feature    2186..2265
                     /label=BBa_B0010
     terminator      2186..2265
                     /label=BBa_B0010
     stem_loop       2281..2300
                     /label=T7 TE
     misc_feature    2307..2307
                     /label=stop
     polyA_site      2301..2314
                     /label=polya
     misc_feature    2274..2314
                     /label=BBa_B0012
     terminator      2274..2314
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
      121 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
      181 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
      241 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
      301 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
      361 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
      421 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
      481 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
      541 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
      601 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
      661 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
      721 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
      781 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
      841 gcaatgcgcg ccattaccga gtccgggctg cgcgttgatg cggatatctc ggtagtggga
      901 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
      961 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     1021 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     1081 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1141 gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa
     1201 taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg
     1261 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1321 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagaattgtg
     1381 agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata ctagagaaag
     1441 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1501 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1561 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1621 tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc
     1681 ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1741 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1801 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1861 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1921 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     1981 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2041 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
     2101 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     2161 agctgtacaa gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     2221 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     2281 ggctcacctt cgggtgggcc tttctgcgtt tata
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