LOCUS       BBa_J06914              1927 bp    DNA     linear       26-Dec-2024
DEFINITION  Lambda cI857 (E07700) QPI regulated by 434 cI with EYFP
ACCESSION   BBa_J06914
VERSION     BBa_J06914.1  
FEATURES             Location/Qualifiers
     misc_feature    1..43
                     /label=OR2
     misc_feature    8..21
                     /label=OR2
     misc_feature    30..43
                     /label=OR1
     promoter        1..6
                     /label=-35
     promoter        24..29
                     /label=-10
     promoter        2..7
                     /label=-35
     misc_feature    35..37
                     /label=start
     misc_feature    1..46
                     /label=BBa_R0052
     promoter        1..46
                     /label=BBa_R0052
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             73..783
                     /label=cI857
     misc_feature    784..816
                     /label=LVA
     misc_feature    320..320
                     /label=G->C
     misc_feature    424..424
                     /label=G->A (ind-)
     misc_feature    753..753
                     /label=C->G
     misc_feature    823..847
                     /label=barcode from lambda cI (BBa_C0051)
     CDS             73..847
                     /label=BBa_J06502
     stem_loop       867..910
                     /label=stem_loop
     misc_feature    856..935
                     /label=BBa_B0010
     terminator      856..935
                     /label=BBa_B0010
     stem_loop       951..970
                     /label=T7 TE
     misc_feature    977..977
                     /label=stop
     polyA_site      971..984
                     /label=polya
     misc_feature    944..984
                     /label=BBa_B0012
     terminator      944..984
                     /label=BBa_B0012
     promoter        1030..1035
                     /label=-10
     promoter        1007..1012
                     /label=-35
     misc_feature    1017..1033
                     /label=OR1
     misc_feature    993..1009
                     /label=OR2
     misc_feature    993..1041
                     /label=BBa_R0051
     promoter        993..1041
                     /label=BBa_R0051
     misc_feature    1054..1057
                     /label=conserved
     RBS             1050..1061
                     /label=BBa_B0034
     CDS             1068..1790
                     /label=BBa_E0030
     stem_loop       1810..1853
                     /label=stem_loop
     misc_feature    1799..1878
                     /label=BBa_B0010
     terminator      1799..1878
                     /label=BBa_B0010
     stem_loop       1894..1913
                     /label=T7 TE
     misc_feature    1920..1920
                     /label=stop
     polyA_site      1914..1927
                     /label=polya
     misc_feature    1887..1927
                     /label=BBa_B0012
     terminator      1887..1927
                     /label=BBa_B0012
ORIGIN
        1 ttgacaaaca agatacattg tatgaaaata caagaaagtt tgttgatact agagaaagag
       61 gagaaatact agatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca
      121 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct
      181 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat
      241 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa
      301 tttagccctt caatcgccac agaaatctac gagatgtatg aagcggttag tatgcagccg
      361 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca
      421 cctaagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa
      481 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc
      541 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag
      601 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc
      661 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc
      721 aatgagagtt gttccgttgt ggggaaagtt atggctagtc agtggcctga agagacgttt
      781 ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt
      841 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      901 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      961 cgggtgggcc tttctgcgtt tatatactag agtaacaccg tgcgtgttga ctattttacc
     1021 tctggcggtg ataatggttg ctactagaga aagaggagaa atactagatg gtgagcaagg
     1081 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1141 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1201 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     1261 tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagctg cacgacttct
     1321 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1381 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1441 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1501 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1561 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1621 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
     1681 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1741 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaataa tactagagcc
     1801 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1861 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1921 gtttata
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